Biomaterial Database

Accessibility of ribosomal proteins to lactoperoxidase-catalyzed iodination following phosphorylation and during subunit interaction. 1978

P W Tas, and B H Sells

Lactoperoxidase-catalyzed iodination was employed as a probe to monitor conformational change in 40-S ribosomal subunits from rat liver. Using this probe, it was observed that phosphorylation of protein S6 resulted in no detectable change in the iodination pattern of 40-S subunit proteins. These results suggest that the conformation of the small subunit remains unaltered following phosphorylation. On the other hand, the differences noted in the iodination pattern between 40-S ribosomal proteins derived from isolated subunits and those from 80-S monosomes, suggest that the 40-S subunit undergoes a conformational change during association with the 60-S subunit. Following 40-S and 60-S subunit association, proteins S2, S3, S5, S6, S8, S10 and S14 became less accessible to iodination. It is suggested that these proteins may be located at the interface between the 40-S and 60-S subunits.

UI	MeSH Term	Description	Entries
D007457	Iodine Radioisotopes	Unstable isotopes of iodine that decay or disintegrate emitting radiation. I atoms with atomic weights 117-139, except I 127, are radioactive iodine isotopes.	Radioisotopes, Iodine
D007553	Isotope Labeling	Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.	Isotope Labeling, Stable,Isotope-Coded Affinity Tagging,Isotopically-Coded Affinity Tagging,Affinity Tagging, Isotope-Coded,Affinity Tagging, Isotopically-Coded,Isotope Coded Affinity Tagging,Labeling, Isotope,Labeling, Stable Isotope,Stable Isotope Labeling,Tagging, Isotope-Coded Affinity,Tagging, Isotopically-Coded Affinity
D007784	Lactoperoxidase	An enzyme derived from cow's milk. It catalyzes the radioiodination of tyrosine and its derivatives and of peptides containing tyrosine.
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008115	Liver Regeneration	Repair or renewal of hepatic tissue.	Liver Regenerations,Regeneration, Liver,Regenerations, Liver
D010446	Peptide Fragments	Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.	Peptide Fragment,Fragment, Peptide,Fragments, Peptide
D010766	Phosphorylation	The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.	Phosphorylations
D011487	Protein Conformation	The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).	Conformation, Protein,Conformations, Protein,Protein Conformations
D002700	Chloramines	Inorganic derivatives of ammonia by substitution of one or more hydrogen atoms with chlorine atoms or organic compounds with the general formulas R2NCl and RNCl2 (where R is an organic group).	Chloroamines
D005260	Female		Females