BIOMAT Database Logo BIOMAT Database Logo

Physical, chemical, and biological factors affecting sister-chromatid exchange induction in human lymphocytes exposed to mitomycin C prior to culture. 1981

L G Littlefield, and S P Colyer, and R J DuFrain

In experiments to assess the effects of several biological, chemical, and physical variables on sister-chromatid exchange (SCE) induction in cultured lymphocytes exposed to mitomycin C (MMC) before PHA stimulation we observed: (1) high SCE frequencies in female cells, and normal SCE frequencies in Y-bearing metaphases in mixed cultures containing equal numbers of MMC-treated female lymphocytes and untreated male lymphocytes; (2) small, but statistically significant, decreases in SCEs with increasing pH after G0 exposure in the pH range 6.6-7.6; (3) pronounced reductions in MMC-induced SCEs in lymphocytes exposed at 4 degrees C vs. 37 degrees C. In other studies, SCE induction was evaluated in cultures exposed during G0 to MMC concentrations ranging from 0.25 to 2.5 microgram/ml for varying time intervals ranging from 5 min to 24 h. For all concentrations tested SCE induction varied as a linear function of G0 exposure time. To compare SCE induction between cultures, we calculated the mean frequencies of SCEs induced per metaphase/unit dose MMC/unit G0 exposure time (SCE/microgram/h). A mean frequency of 20.7 +/- 4.8 SCE/microgram/h was observed for 41 lymphocyte cultures suggesting that a single term adequately describes the rate of SCE induction following G0 exposure to a 10-fold range in concentration of MMC for time intervals of 30 min to 24 h.

UI MeSH Term Description Entries
D008214 Lymphocytes White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS. Lymphoid Cells,Cell, Lymphoid,Cells, Lymphoid,Lymphocyte,Lymphoid Cell
D008297 Male Males
D008937 Mitomycins A group of methylazirinopyrroloindolediones obtained from certain Streptomyces strains. They are very toxic antibiotics used as ANTINEOPLASTIC AGENTS in some solid tumors. PORFIROMYCIN and MITOMYCIN are the most useful members of the group.
D002478 Cells, Cultured Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others. Cultured Cells,Cell, Cultured,Cultured Cell
D003434 Crossing Over, Genetic The reciprocal exchange of segments at corresponding positions along pairs of homologous CHROMOSOMES by symmetrical breakage and crosswise rejoining forming cross-over sites (HOLLIDAY JUNCTIONS) that are resolved during CHROMOSOME SEGREGATION. Crossing-over typically occurs during MEIOSIS but it may also occur in the absence of meiosis, for example, with bacterial chromosomes, organelle chromosomes, or somatic cell nuclear chromosomes. Crossing Over,Crossing-Over, Genetic,Crossing Overs,Genetic Crossing Over,Genetic Crossing-Over
D003470 Culture Media Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN. Media, Culture
D004305 Dose-Response Relationship, Drug The relationship between the dose of an administered drug and the response of the organism to the drug. Dose Response Relationship, Drug,Dose-Response Relationships, Drug,Drug Dose-Response Relationship,Drug Dose-Response Relationships,Relationship, Drug Dose-Response,Relationships, Drug Dose-Response
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863 Hydrogen-Ion Concentration The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations

Related Publications

L G Littlefield, and S P Colyer, and R J DuFrain
Induction of 8-azaguanine resistance and sister chromatid exchange in human lymphocytes exposed to mitomycin C and X rays in vitro.
August 1981, Nature,
L G Littlefield, and S P Colyer, and R J DuFrain
Induction of long-lived chromosome damage, as manifested by sister-chromatid exchange, in lymphocytes of animals exposed to mitomycin-C.
September 1978, Mutation research,
L G Littlefield, and S P Colyer, and R J DuFrain
Sister chromatid exchange induction in human lymphocytes exposed to benzene and its metabolites in vitro.
June 1985, Cancer research,
L G Littlefield, and S P Colyer, and R J DuFrain
Broccoli flower head extract reduces mitomycin-C induced sister chromatid exchange in cultured human lymphocytes.
October 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association,
L G Littlefield, and S P Colyer, and R J DuFrain
Induction of sister chromatid exchanges by mitomycin C in lymphocytes of young and old human donors.
January 1984, Gerontology,
L G Littlefield, and S P Colyer, and R J DuFrain
Sister-chromatid exchange induction by mitomycin C is deficient in embryonal carcinoma cells.
November 1979, Mutation research,
L G Littlefield, and S P Colyer, and R J DuFrain
Factors influencing the frequency of mitomycin C-induced sister-chromatid exchanges in 5-bromodeoxyuridine-substituted human lymphocytes in culture.
September 1978, Mutation research,
L G Littlefield, and S P Colyer, and R J DuFrain
Sister chromatid exchange in human lymphocytes exposed to isoflurane and nitrous oxide in vitro.
February 1999, British journal of anaesthesia,
L G Littlefield, and S P Colyer, and R J DuFrain
[The induction of sister chromatid exchange by diethylstilbestrol and mitomycin C in lymphocytes of mothers and their newborn infants].
April 1987, Zentralblatt fur Bakteriologie, Mikrobiologie und Hygiene. Serie B, Umwelthygiene, Krankenhaushygiene, Arbeitshygiene, praventive Medizin,
L G Littlefield, and S P Colyer, and R J DuFrain
Factors influencing sister-chromatid exchange rate in cultured human lymphocytes.
May 1981, Mutation research,
Copied contents to your clipboard!