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A highly sensitive thin layer chromatographic method for the separation of porphyrin fractions and calculation of their percentage ratio was put into practice for the first time in our country. It was elaborated on the base of Doss method with some modifications. It enables the separation of the various porphyrin fractions as porphyrinmethyl esters in urine, feces, serum and liver. The chromatograms were directly read by a fluorescent densitometer MPF--43, with an appliance for thin layer chromatographic plaques (Hitachi). The method is with a valuable clinical significance in the diagnostics and follow-up of the patients with porphyria and in the studies on the disturbances in porphyrin metabolism.
R Tanev, and
E Ivanov
December 1986,
Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology,
R Tanev, and
E Ivanov
May 1970,
Zeitschrift fur klinische Chemie und klinische Biochemie,
R Tanev, and
E Ivanov
July 1971,
Journal of chromatography,
R Tanev, and
E Ivanov
December 1994,
Talanta,
R Tanev, and
E Ivanov
May 1969,
Journal of chromatography,
R Tanev, and
E Ivanov
May 1967,
South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde,
R Tanev, and
E Ivanov
December 1968,
Analytical biochemistry,
R Tanev, and
E Ivanov
October 1989,
Analytical chemistry,
R Tanev, and
E Ivanov
October 1969,
Journal of chromatography,
R Tanev, and
E Ivanov
September 1975,
The British journal of dermatology,
