Biomaterial Database

Direct spectrophotometric determination of serum and urinary oxalate with oxalate oxidase. 1981

G Kohlbecker, and M Butz

A new enzymatic method for direct photometric determination of oxalate in serum and urine is described, using oxalate oxidase. The resulting H2O2 is measured with a coupled enzyme system of catalase and aldehyde dehydrogenase. Percentage recovery of added oxalate was 99 +/- 4 in serum, and 98 +/- 4 in urine (n - 10). Oxalate serum levels varied from 16.9 to 44.8 mumol/l. Oxalate values can be determined within 20 minutes, without time consuming pretreatment of samples. The detection limit is 5 mumol/l.

UI	MeSH Term	Description	Entries
D008832	Microchemistry	The development and use of techniques and equipment to study or perform chemical reactions, with small quantities of materials, frequently less than a milligram or a milliliter.
D010070	Oxalates	Derivatives of OXALIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that are derived from the ethanedioic acid structure.	Oxalate,Ethanedioic Acids,Oxalic Acids,Acids, Ethanedioic,Acids, Oxalic
D010088	Oxidoreductases	The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)	Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D002374	Catalase	An oxidoreductase that catalyzes the conversion of HYDROGEN PEROXIDE to water and oxygen. It is present in many animal cells. A deficiency of this enzyme results in ACATALASIA.	Catalase A,Catalase T,Manganese Catalase,Mn Catalase
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006861	Hydrogen Peroxide	A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.	Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D000444	Aldehyde Dehydrogenase	An enzyme that oxidizes an aldehyde in the presence of NAD+ and water to an acid and NADH. This enzyme was formerly classified as EC 1.1.1.70.	D-Glucuronolactone Dehydrogenase,Aldehyde Dehydrogenase (NAD(+)),Aldehyde Dehydrogenase E1,Aldehyde Dehydrogenase E2,Aldehyde-NAD Oxidoreductase,Aldehyde NAD Oxidoreductase,D Glucuronolactone Dehydrogenase,Dehydrogenase, Aldehyde,Dehydrogenase, D-Glucuronolactone
D000445	Aldehyde Oxidoreductases	Oxidoreductases that are specific for ALDEHYDES.	Aldehyde Oxidoreductase,Oxidoreductase, Aldehyde,Oxidoreductases, Aldehyde
D013053	Spectrophotometry	The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
D019815	Oxalic Acid	A strong dicarboxylic acid occurring in many plants and vegetables. It is produced in the body by metabolism of glyoxylic acid or ascorbic acid. It is not metabolized but excreted in the urine. It is used as an analytical reagent and general reducing agent.	Aluminum Oxalate,Ammonium Oxalate,Chromium (2+) Oxalate,Chromium (3+) Oxalate (3:2),Chromium Oxalate,Diammonium Oxalate,Dilithium Oxalate,Dipotassium Oxalate,Disodium Oxalate,Ferric Oxalate,Iron (2+) Oxalate (1:1),Iron (3+) Oxalate,Iron Oxalate,Magnesium Oxalate,Magnesium Oxalate (1:1),Manganese (2+) Oxalate (1:1),Monoammonium Oxalate,Monohydrogen Monopotassium Oxalate,Monopotassium Oxalate,Monosodium Oxalate,Potassium Chromium Oxalate,Potassium Oxalate,Potassium Oxalate (2:1),Sodium Oxalate,Acid, Oxalic,Oxalate, Aluminum,Oxalate, Chromium,Oxalate, Diammonium,Oxalate, Dilithium,Oxalate, Dipotassium,Oxalate, Disodium,Oxalate, Ferric,Oxalate, Iron,Oxalate, Magnesium,Oxalate, Monoammonium,Oxalate, Monohydrogen Monopotassium,Oxalate, Monopotassium,Oxalate, Monosodium,Oxalate, Potassium,Oxalate, Potassium Chromium,Oxalate, Sodium