Biomaterial Database

Determination of urinary oxalate with oxalate oxidase and peroxidase immobilized on to glass beads. 1998

C S Pundir, and N K Kuchhal, and A K Bhargava

Department of Bio Sciences, Maharshi Dayanand University, Haryana, India.

We have reported the immobilization of barley oxalate oxidase on to alkylamine glass beads through glutaraldehyde coupling (Pundir, C. S., Satyapal and Kuchhal, N. K. (1993) Clinical Chemistry 39, 1750-1751). The present report describes the immobilization of commercially available horseradish peroxidase on to zirconia-coated arylamine glass beads through diazotization and a new method for the discrete assay of urinary oxalate using both immobilized oxalate oxidase and peroxidase. In the method, urinary oxalate is precipitated with CaCl2, redissolved in HCl and then assayed using immobilized enzymes. The oxalate in 24 h urine samples from apparently healthy male adults was measured by this method and found to be in the range of 12.2-28.0 mg with a mean of 19.8 mg. The percentage recovery of added oxalate (17.5 mg/l) was 96.7 +/- 3.4 (mean +/- SD). The mean value of urinary oxalate by our method is comparable with those obtained by the Sigma kit method. The cost of oxalate determination in 100 urine samples by the present method has been compared with that of the Sigma kit method.

UI	MeSH Term	Description	Entries
D008297	Male		Males
D010070	Oxalates	Derivatives of OXALIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that are derived from the ethanedioic acid structure.	Oxalate,Ethanedioic Acids,Oxalic Acids,Acids, Ethanedioic,Acids, Oxalic
D010088	Oxidoreductases	The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)	Dehydrogenases,Oxidases,Oxidoreductase,Reductases,Dehydrogenase,Oxidase,Reductase
D012016	Reference Values	The range or frequency distribution of a measurement in a population (of organisms, organs or things) that has not been selected for the presence of disease or abnormality.	Normal Range,Normal Values,Reference Ranges,Normal Ranges,Normal Value,Range, Normal,Range, Reference,Ranges, Normal,Ranges, Reference,Reference Range,Reference Value,Value, Normal,Value, Reference,Values, Normal,Values, Reference
D004800	Enzymes, Immobilized	Enzymes which are immobilized on or in a variety of water-soluble or water-insoluble matrices with little or no loss of their catalytic activity. Since they can be reused continuously, immobilized enzymes have found wide application in the industrial, medical and research fields.	Immobilized Enzymes,Enzyme, Immobilized,Immobilized Enzyme
D006735	Horseradish Peroxidase	An enzyme isolated from horseradish which is able to act as an antigen. It is frequently used as a histochemical tracer for light and electron microscopy. Its antigenicity has permitted its use as a combined antigen and marker in experimental immunology.	Alpha-Peroxidase,Ferrihorseradish Peroxidase,Horseradish Peroxidase II,Horseradish Peroxidase III,Alpha Peroxidase,II, Horseradish Peroxidase,III, Horseradish Peroxidase,Peroxidase II, Horseradish,Peroxidase III, Horseradish,Peroxidase, Ferrihorseradish,Peroxidase, Horseradish
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D001467	Hordeum	A plant genus of the family POACEAE. The EDIBLE GRAIN, barley, is widely used as food.	Barley,Hordeum vulgare
D001671	Biochemistry	The study of the composition, chemical structures, and chemical reactions of living things.
D013997	Time Factors	Elements of limited time intervals, contributing to particular results or situations.	Time Series,Factor, Time,Time Factor