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A method has been developed for the rapid separation of cells in suspension from non-cell associated lipid vesicles in various assays for vesicle-cell interation. Separation is achieved on a discontinuous Ficoll-Paque gradient. Cells and free vesicles are totally separated, as evidenced by both radiolabelled vesicles, and vesicles containing the fluorescent dye 6-carboxyfluorescein. The main advantages of this method are the rapidity, efficacy, and gentleness of the separation. Viability of the cells remains consistently high (greater than 96%) throughout the separation. Since this method involves a one-step centrifugation, it precludes the necessity for repeated washings of cells which have been incubated with lipid vesicles.
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
September 2019,
BioTechniques,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
January 1986,
Oncology,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
February 1984,
Bulletin of environmental contamination and toxicology,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
February 1971,
Nature,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
August 1980,
The Australian journal of experimental biology and medical science,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
June 1987,
Plant physiology,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
January 1994,
BioTechniques,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
June 1964,
Virology,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
January 1979,
Developments in biological standardization,
M W Fountain, and
R Chiovetti, and
H Kercret, and
D O Parrish, and
J P Segrest
September 1994,
JAMA,
