-transparent.png){kind=logo}
The selective removal of nonviable cells from suspension cultures of lymphoid cell lines by buoyant density centrifugation at 400 x g on a Ficoll-Hypaque (FH) cushion is described. Viable cells collect at the medium-FH interface whereas nonviable cells pellet. This method has proven useful in separating viable from nonviable cells with human lymphoblastoid cell lines, rabbit lymphoid cell lines and mouse myeloma and hybridoma cell lines.
Related Publications
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
March 1980,
Biochimica et biophysica acta,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
April 1973,
Journal of immunological methods,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
June 1975,
Journal of immunological methods,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
October 1987,
American journal of medical genetics,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
June 1987,
Plant physiology,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
January 1994,
BioTechniques,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
November 1979,
FEBS letters,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
April 1994,
Journal of virological methods,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
January 1980,
Journal of immunological methods,
J G Hurrell, and
P H Black, and
E Haber, and
V R Zurawski
October 1963,
Folia biologica,
