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1. The transbilayer distribution of the phospholipids in sonicated egg phosphatidylcholine-phosphatidic acid vesicles and the interaction of Ca2+ with these vesicles was studied by 31P NMR. 2. Over a wide composition range the bilayer of these vesicles has a symmetrical phospholipid composition. 3. With ratios of Ca2+ to phosphatidic acid in the outer monolayer of the vesicles up to 0.3, Ca2+ induces vesicle aggregation. The extent of aggregation is increased by the Ca2+ concentration in the medium and the outer monolayer concentration of phosphatidic acid. The vesicle aggregation can be fully reversed by chelating Ca2+. 4. When the ratio exceeds 0.5 Ca2+ induces vesicle fusion. The fusion is maximal for vesicles containing both phosphatidylcholine and phosphatidic acid. The data suggest that Ca2+-induced lateral phase separations make the bilayer more susceptible to fusion.
M Koter, and
B de Kruijff, and
L L van Deenen
January 1979,
Biochimica et biophysica acta,
M Koter, and
B de Kruijff, and
L L van Deenen
October 1980,
Biochimica et biophysica acta,
M Koter, and
B de Kruijff, and
L L van Deenen
March 1985,
Biochimica et biophysica acta,
M Koter, and
B de Kruijff, and
L L van Deenen
October 1979,
Biochemical and biophysical research communications,
M Koter, and
B de Kruijff, and
L L van Deenen
November 1986,
Biochemistry,
M Koter, and
B de Kruijff, and
L L van Deenen
April 1988,
Chemistry and physics of lipids,
M Koter, and
B de Kruijff, and
L L van Deenen
June 1980,
Biochimica et biophysica acta,
M Koter, and
B de Kruijff, and
L L van Deenen
August 1978,
Archives of biochemistry and biophysics,
M Koter, and
B de Kruijff, and
L L van Deenen
March 1985,
Biochimica et biophysica acta,
M Koter, and
B de Kruijff, and
L L van Deenen
April 1988,
Biochimica et biophysica acta,
