Biomaterial Database

Fractionation and characterization of rat hepatocytes isolated from ethanol-induced fatty liver. 1980

J Kondrup, and B Bro, and J Dich, and N Grunnet, and H I Thieden

A procedure for fractionation of hepatocytes according to density is presented. Hepatocytes were prepared from rats fed an ethanol-containing liquid diet, a liquid diet without ethanol, or a chow diet. The hepatocytes were fractionated in a Metrizamide density gradient and the cell fractions obtained were characterized with respect to protein synthesis, fatty acid oxidation, and esterification and ATP content. From ethanol-fed rats, two fractions of hepatocytes were obtained, one with a density of approximately 1.04 gm. per ml. and one with a density of ca. 1.08 gm. per ml. Cells from the lighter fraction contained 3 times more triacylglycerol than cells from the heavier fraction. Fifty per cent of the cells from the lighter fraction contained large (greater than 5 micron) lipid droplets as compared to 6% in the heavier fraction. The average yield from a 170-gm. rat of lipid-rich and lipid-poor cells was about 65 x 10(6) and 170 x 10(6) cells, respectively. Electron micrographs of the liver before perfusion and of the isolated and washed cell fraction revealed intact ultrastructure. Fractionation of hepatocyte suspensions from rats fed a high fat liquid diet without ethanol or a chow diet normally yielded two cell fractions with densities of ca. 1.08 and 1.10 gm. per ml. and 1.10 and 1.12 gm. per ml., respectively. The metabolic capacity of the cell fractions was unaltered by the fractionation procedure as judged by the ATP content, the rate of palmitate oxidation and esterification, and the rate of protein synthesis.

UI	MeSH Term	Description	Entries
D008099	Liver	A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.	Livers
D008112	Liver Glycogen	Glycogen stored in the liver. (Dorland, 28th ed)	Hepatic Glycogen,Glycogen, Hepatic,Glycogen, Liver
D010743	Phospholipids	Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.	Phosphatides,Phospholipid
D011506	Proteins	Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.	Gene Products, Protein,Gene Proteins,Protein,Protein Gene Products,Proteins, Gene
D002469	Cell Separation	Techniques for separating distinct populations of cells.	Cell Isolation,Cell Segregation,Isolation, Cell,Cell Isolations,Cell Segregations,Cell Separations,Isolations, Cell,Segregation, Cell,Segregations, Cell,Separation, Cell,Separations, Cell
D002499	Centrifugation, Density Gradient	Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Centrifugations, Density Gradient,Density Gradient Centrifugation,Density Gradient Centrifugations,Gradient Centrifugation, Density,Gradient Centrifugations, Density
D005227	Fatty Acids	Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)	Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D005235	Fatty Liver, Alcoholic	Lipid infiltration of the hepatic parenchymal cells that is due to ALCOHOL ABUSE. The fatty changes in the alcoholic fatty liver may be reversible, depending on the amounts of TRIGLYCERIDES accumulated.	Alcoholic Fatty Liver,Alcoholic Steatohepatitis
D005260	Female		Females
D000255	Adenosine Triphosphate	An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.	ATP,Adenosine Triphosphate, Calcium Salt,Adenosine Triphosphate, Chromium Salt,Adenosine Triphosphate, Magnesium Salt,Adenosine Triphosphate, Manganese Salt,Adenylpyrophosphate,CaATP,CrATP,Manganese Adenosine Triphosphate,MgATP,MnATP,ATP-MgCl2,Adenosine Triphosphate, Chromium Ammonium Salt,Adenosine Triphosphate, Magnesium Chloride,Atriphos,Chromium Adenosine Triphosphate,Cr(H2O)4 ATP,Magnesium Adenosine Triphosphate,Striadyne,ATP MgCl2