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An improved method for the homogenization and the subsequent subcellular fractionation of hepatocytes isolated from adult rat liver is described. The homogenization procedure developed in the present study allows the preservation of the integrity of subcellular structures, as demonstrated by measurement of the activities of representative enzymes as well as by determination of their latency. The activities of representative marker enzymes, as calculated on subcellular fractions obtained by differential centrifugation of the homogenate, are identical whether the homogenate arises from isolated hepatocytes or from the whole liver. Moreover, there is a close similitude between the kinetic parameters (Km and V) of two microsomal cytochrome P450-dependent mixed-function oxidases, namely aniline hydroxylase and aminopyrine demethylase determined on microsomal preparations obtained either from isolated cells or from the whole liver.
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
January 1980,
Biochemical and biophysical research communications,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
January 1974,
Methods in enzymology,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
October 1974,
Hoppe-Seyler's Zeitschrift fur physiologische Chemie,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
January 1993,
Sub-cellular biochemistry,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
May 1975,
Archives internationales de physiologie et de biochimie,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
January 1982,
Antibiotiki,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
August 1980,
Laboratory investigation; a journal of technical methods and pathology,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
February 1980,
The Biochemical journal,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
November 1978,
Clinical science and molecular medicine,
G Krack, and
O Gravier, and
M Roberfroid, and
M Mercier
January 1977,
Drug metabolism and disposition: the biological fate of chemicals,
