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Aliquots of a solution of highly purified human pituitary LH (hLH) were incubated with variations in temperature and time. The incubates were chromatographed on Sephadex G-100 as well as on DEAE-Sephadex A25. The column effluents were assayed in radioligand assay and in specific hLH alpha- and hLH beta-RIA systems. The results indicate that there is spontaneous dissociation of hLH at elevated temperatures under otherwise "normal" conditions concerning, for example, pH and ionic strength. The degree of dissociation is both time and temperature dependent. This dissociation is not due to proteolytic enzymes. It is concluded that one should be alert when using hLH subunit RIA systems at elevated temperatures for measuring hLH levels, e.g. in clinical samples, because artificial high levels may be obtained.
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
February 1969,
Biochemistry,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
September 1978,
Journal of neurosurgery,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
November 1978,
Molecular and cellular endocrinology,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
April 1975,
The Journal of biological chemistry,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
December 1963,
Experimental cell research,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
February 1966,
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
April 1970,
The Journal of endocrinology,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
December 1971,
The Journal of biological chemistry,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
November 1974,
The Journal of clinical endocrinology and metabolism,
J G Loeber, and
J W Nabben-Fleuren, and
L H Elvers, and
M F Segers, and
R M Lequin
September 1971,
Clinical chemistry,
