BIOMAT Database Logo BIOMAT Database Logo

Trypsinogen expression in human ovarian carcinomas. 1995

F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, Japan.

Increased secretion of matrix metalloproteinases and serine proteinases is well known to be associated with cancer invasion and metastasis. We aimed to elucidate the implication of trypsin, a serine proteinase and a representative digestive enzyme in invasion and metastasis of human carcinomas. Northern blot, RT-PCR and Western blot analyses and immunohistochemical studies were performed to detect and analyze trypsinogen expression in 5 ovarian carcinoma cell lines and 10 human ovarian carcinoma tissues using a DNA probe for trypsinogen I, and monoclonal and polyclonal antibodies to human trypsin I. Among the 5 ovarian carcinoma cell lines, only the MCAS (mucinous cystadenocarcinoma) cell line showed a high level of trypsinogen production and mRNA expression by Western and Northern blot analyses, respectively. However, Southern blot analysis of RT-PCR products could detect considerable levels of trypsinogen mRNA in all ovarian cancer cell lines. In Northern analysis of ovarian cancer tissues, all advanced cancer samples showed trypsinogen gene expression. Serous cystadenocarcinomas exhibited particularly high levels of gene expression. Immunohistochemical staining also detected trypsin in ovarian carcinoma tissues. In contrast, normal ovaries and tumors with low malignant potential did not show trypsinogen expression. Our results demonstrate the extra-pancreatic production and distribution of trypsinogen in human ovarian carcinomas.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009362 Neoplasm Metastasis The transfer of a neoplasm from one organ or part of the body to another remote from the primary site. Metastase,Metastasis,Metastases, Neoplasm,Metastasis, Neoplasm,Neoplasm Metastases,Metastases
D010051 Ovarian Neoplasms Tumors or cancer of the OVARY. These neoplasms can be benign or malignant. They are classified according to the tissue of origin, such as the surface EPITHELIUM, the stromal endocrine cells, and the totipotent GERM CELLS. Cancer of Ovary,Ovarian Cancer,Cancer of the Ovary,Neoplasms, Ovarian,Ovary Cancer,Ovary Neoplasms,Cancer, Ovarian,Cancer, Ovary,Cancers, Ovarian,Cancers, Ovary,Neoplasm, Ovarian,Neoplasm, Ovary,Neoplasms, Ovary,Ovarian Cancers,Ovarian Neoplasm,Ovary Cancers,Ovary Neoplasm
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000230 Adenocarcinoma A malignant epithelial tumor with a glandular organization. Adenocarcinoma, Basal Cell,Adenocarcinoma, Granular Cell,Adenocarcinoma, Oxyphilic,Adenocarcinoma, Tubular,Adenoma, Malignant,Carcinoma, Cribriform,Carcinoma, Granular Cell,Carcinoma, Tubular,Adenocarcinomas,Adenocarcinomas, Basal Cell,Adenocarcinomas, Granular Cell,Adenocarcinomas, Oxyphilic,Adenocarcinomas, Tubular,Adenomas, Malignant,Basal Cell Adenocarcinoma,Basal Cell Adenocarcinomas,Carcinomas, Cribriform,Carcinomas, Granular Cell,Carcinomas, Tubular,Cribriform Carcinoma,Cribriform Carcinomas,Granular Cell Adenocarcinoma,Granular Cell Adenocarcinomas,Granular Cell Carcinoma,Granular Cell Carcinomas,Malignant Adenoma,Malignant Adenomas,Oxyphilic Adenocarcinoma,Oxyphilic Adenocarcinomas,Tubular Adenocarcinoma,Tubular Adenocarcinomas,Tubular Carcinoma,Tubular Carcinomas
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA
D012333 RNA, Messenger RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm. Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D012334 RNA, Neoplasm RNA present in neoplastic tissue. Neoplasm RNA
D014407 Tumor Cells, Cultured Cells grown in vitro from neoplastic tissue. If they can be established as a TUMOR CELL LINE, they can be propagated in cell culture indefinitely. Cultured Tumor Cells,Neoplastic Cells, Cultured,Cultured Neoplastic Cells,Cell, Cultured Neoplastic,Cell, Cultured Tumor,Cells, Cultured Neoplastic,Cells, Cultured Tumor,Cultured Neoplastic Cell,Cultured Tumor Cell,Neoplastic Cell, Cultured,Tumor Cell, Cultured

Related Publications

F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Expression of pancreatic trypsinogen in human extrapancreatic gastrointestinal carcinomas.
July 1994, Oncology reports,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Expression of pancreatic trypsinogen/trypsin and cathepsin B in human cholangiocarcinomas and hepatocellular carcinomas.
July 1995, Human pathology,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Loss of Gelsolin expression in human ovarian carcinomas.
February 2005, European journal of cancer (Oxford, England : 1990),
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Expression of the DF3-P epitope in human ovarian carcinomas.
May 1995, Clinical cancer research : an official journal of the American Association for Cancer Research,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Expression of a human multidrug resistance gene in ovarian carcinomas.
September 1989, Cancer research,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Differential expression of pancreatic trypsinogen and cathepsin-B in human scirrhous-type and intestinal-type gastric carcinomas.
January 1994, Oncology reports,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Gene expression patterns in ovarian carcinomas.
November 2003, Molecular biology of the cell,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Survivin expression in 49 ovarian carcinomas.
May 2004, Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Expression of p73 and c-Abl proteins in human ovarian carcinomas.
June 2003, Journal of Nippon Medical School = Nippon Ika Daigaku zasshi,
F Hirahara, and Y Miyagi, and E Miyagi, and H Yasumitsu, and N Koshikawa, and Y Nagashima, and H Kitamura, and H Minaguchi, and M Umeda, and K Miyazaki
Expression analysis of EGF-R and TGFa in human ovarian carcinomas.
January 1991, Anticancer research,
Copied contents to your clipboard!