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A nucleolar chromatin antigen (NoAg-1) found in Novikoff hepatoma but not in normal liver has been purified to homogeneity as shown by two-dimensional gel electrophoresis. Initial purification of NoAg-1 was partially achieved by isolation of nucleolar chromatin and fractionation of its proteins by successive extraction with solutions of increasing salt concentration. Further purification of this antigen was achieved by affinity and hydroxylapatite chromatography. Although approximately 50% of the NoAg-1 antigen was in the 0.6 M NaCl extract of Novikoff nucleoli, it was less pure than in the 2 M NaCl:5 M urea extract which contained 25% of the NoAg-1 at a purity of 40%. The highly purified NoAg-1 had an approximate molecular weight of 60,000 and pl of 5.1; the yield of NoAg-1 was 0.22% of the total nucleolar proteins.
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
September 1978,
Biochimica et biophysica acta,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
September 1973,
Biochimica et biophysica acta,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
May 1971,
Biochimica et biophysica acta,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
January 1978,
The International journal of biochemistry,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
May 1980,
Cancer research,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
December 1984,
Cancer research,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
January 1971,
The Journal of biological chemistry,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
July 1988,
Nucleic acids research,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
April 1978,
Proceedings of the National Academy of Sciences of the United States of America,
F Marashi, and
F M Davis, and
R K Busch, and
H E Savage, and
H Busch
January 1977,
Tumori,
