Biomaterial Database

Radiocontrast media induced endothelin-1 mRNA expression and peptide release in porcine aortic endothelial cells. 1995

J M Sung, and G H Shu, and J C Tsai, and J J Huang

Department of Internal Medicine, National Cheng Kung University Hospital, Tainan, Taiwan, R.O.C.

Endothelin-1 (ET-1) is a powerful vasoconstrictor, primarily secreted by vascular endothelial cells. It may be an important mediator for radiocontrast-induced renal vasoconstriction, which may cause acute renal failure in susceptible subjects. This study measured the release of ET-1 by cultured porcine endothelial cells under incubation with various radiocontrast agents. Compared with the control group, the conventional ionic radiocontrast, diatrizoate, induced a dose- and time-dependent increase in ET-1 release. The lowest concentration of diatrizoate required to induce an increase in ET-1 secretion was 1 mM, less than the estimated serum level (about 5.2 mM) in clinical radiographic practice, suggesting that a direct release of ET-1 from endothelial cells may take place in clinical image studies. The stimulation effect of the following contrast agents on endothelial ET-1 release was compared: diatrizoate (ionic monomer), ioxaglate (ionic dimer), iohexol (nonionic monomer), iotrolan (nonionic dimer). Higher levels of ET-1 release were stimulated by ionic agents compared to the nonionic agents, but no difference in the degree of stimulation was observed between the monomeric and dimeric forms. All agents significantly increased ET-1 release compared with the control group. ET-1 production stimulated by radiocontrast agents was independent of hyperosmolality and was primarily seen as an increase in ET-1 mRNA. The increase in ET-1 release was actinomycin D-sensitive, and ET-1 mRNA abundance was greatly enhanced by cycloheximide. The augmented ET-1 mRNA expression to radiocontrast treatment was not secondary to the enhanced message stability. The data suggest that the contrast media-induced increments in ET-1 release are transcription-dependent and that both ionic and nonionic radiocontrast agents have a renal vasoconstrictive effect that is mediated by ET-1 and may potentially be nephrotoxic in susceptible subjects. Nonionic agents may be less toxic.

UI	MeSH Term	Description	Entries
D002478	Cells, Cultured	Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.	Cultured Cells,Cell, Cultured,Cultured Cell
D003287	Contrast Media	Substances used to allow enhanced visualization of tissues.	Radiopaque Media,Contrast Agent,Contrast Agents,Contrast Material,Contrast Materials,Radiocontrast Agent,Radiocontrast Agents,Radiocontrast Media,Agent, Contrast,Agent, Radiocontrast,Agents, Contrast,Agents, Radiocontrast,Material, Contrast,Materials, Contrast,Media, Contrast,Media, Radiocontrast,Media, Radiopaque
D004730	Endothelium, Vascular	Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.	Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001011	Aorta	The main trunk of the systemic arteries.	Aortas
D012333	RNA, Messenger	RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.	Messenger RNA,Messenger RNA, Polyadenylated,Poly(A) Tail,Poly(A)+ RNA,Poly(A)+ mRNA,RNA, Messenger, Polyadenylated,RNA, Polyadenylated,mRNA,mRNA, Non-Polyadenylated,mRNA, Polyadenylated,Non-Polyadenylated mRNA,Poly(A) RNA,Polyadenylated mRNA,Non Polyadenylated mRNA,Polyadenylated Messenger RNA,Polyadenylated RNA,RNA, Polyadenylated Messenger,mRNA, Non Polyadenylated
D013552	Swine	Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).	Phacochoerus,Pigs,Suidae,Warthogs,Wart Hogs,Hog, Wart,Hogs, Wart,Wart Hog
D016232	Endothelins	21-Amino-acid peptides produced by vascular endothelial cells and functioning as potent vasoconstrictors. The endothelin family consists of three members, ENDOTHELIN-1; ENDOTHELIN-2; and ENDOTHELIN-3. All three peptides contain 21 amino acids, but vary in amino acid composition. The three peptides produce vasoconstrictor and pressor responses in various parts of the body. However, the quantitative profiles of the pharmacological activities are considerably different among the three isopeptides.	Endothelium-Derived Vasoconstrictor Factors,Endothelin,Vasoconstrictor Factors, Endothelium-Derived