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Freshly isolated hepatocytes of the clawed toad, Xenopus laevis, were cultured for at least 3 days. The viability of the cells was characterized using staining and biochemical methods. In particular, the glucose and glycogen balance was tested. After culture for 16-20 hrs, the cells were subjected to hormonal treatment. Both adrenaline and arginine vasotocin stimulated the release of glucose in a dose dependent manner. 10(-6) M concentrations were strongly effective. The determination of the glycogen balance made it clear that the glucose release is mainly due to glycogenolysis. Using receptor antagonists and agonists, it has been shown that the effect of adrenaline is clearly mediated by beta-type receptors. Arginine vasotocin stimulated glycogenolysis via a type of receptor which is similar to the V2-receptor of mammals. This means that cAMP is involved in the response to both types of hormones which is in contrast to that which is known about the effect of nonapeptides on the liver of mammals.
T Ade, and
H Segner, and
W Hanke
July 1970,
Comparative biochemistry and physiology,
T Ade, and
H Segner, and
W Hanke
October 1968,
Journal of cellular physiology,
T Ade, and
H Segner, and
W Hanke
March 1964,
Experientia,
T Ade, and
H Segner, and
W Hanke
January 1995,
Zhurnal evoliutsionnoi biokhimii i fiziologii,
T Ade, and
H Segner, and
W Hanke
November 1988,
The Journal of comparative neurology,
T Ade, and
H Segner, and
W Hanke
January 1985,
Folia morphologica,
T Ade, and
H Segner, and
W Hanke
August 1994,
European journal of morphology,
T Ade, and
H Segner, and
W Hanke
January 1965,
Folia haematologica (Leipzig, Germany : 1928),
T Ade, and
H Segner, and
W Hanke
May 1947,
The American journal of medical technology,
T Ade, and
H Segner, and
W Hanke
February 1955,
Nature,
