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A quantitative fluorescent microscopy system was developed to characterize, in real time, the effects of supraphysiological temperatures between 37 degrees and 70 degrees C on the plasma membrane of mouse 3T3 fibroblasts and isolated rat skeletal muscle cells. Membrane permeability was assessed by monitoring the leakage as a function of time of the fluorescent membrane integrity probe calcein. The kinetics of dye leakage increased with increasing temperature in both the 3T3 fibroblasts and the skeletal muscle cells. Analytical solutions derived from a two-compartment transport model showed that, for both cell types, a time-dependent permeability assumption provided a statistically better fit of the model predictions to the data than a constant permeability assumption. This finding suggests that the plasma membrane integrity is continuously being compromised while cells are subjected to supraphysiological temperatures.
J C Bischof, and
J Padanilam, and
W H Holmes, and
R M Ezzell, and
R C Lee, and
R G Tompkins, and
M L Yarmush, and
M Toner
November 1982,
The American journal of physiology,
J C Bischof, and
J Padanilam, and
W H Holmes, and
R M Ezzell, and
R C Lee, and
R G Tompkins, and
M L Yarmush, and
M Toner
January 2004,
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference,
J C Bischof, and
J Padanilam, and
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R M Ezzell, and
R C Lee, and
R G Tompkins, and
M L Yarmush, and
M Toner
June 1966,
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J C Bischof, and
J Padanilam, and
W H Holmes, and
R M Ezzell, and
R C Lee, and
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M Toner
June 1999,
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J C Bischof, and
J Padanilam, and
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R G Tompkins, and
M L Yarmush, and
M Toner
July 1965,
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J C Bischof, and
J Padanilam, and
W H Holmes, and
R M Ezzell, and
R C Lee, and
R G Tompkins, and
M L Yarmush, and
M Toner
October 1950,
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J C Bischof, and
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R C Lee, and
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M L Yarmush, and
M Toner
August 1967,
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J C Bischof, and
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M Toner
May 2019,
Chemical reviews,
J C Bischof, and
J Padanilam, and
W H Holmes, and
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R C Lee, and
R G Tompkins, and
M L Yarmush, and
M Toner
September 1985,
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J C Bischof, and
J Padanilam, and
W H Holmes, and
R M Ezzell, and
R C Lee, and
R G Tompkins, and
M L Yarmush, and
M Toner
November 1999,
Biochimica et biophysica acta,
