Biomaterial Database

Evidence for a conformational change in tRNAPhe upon aminoacylation. 1976

M Caron, and N Brisson, and H Dugas

A conformational difference between the structure of tRNAPhe and Cbz-Phe-tRNAPhe from Escherichia coli was detected using the spin label method. A comparison of the respective rotational correlation time (tau c) values of three differently located spin labels, indicates that upon aminoacylation of tRNAPhe, the 4-thiouridine residue region and the miniloop region become more flexible, while the environment of the anticodon loop is not affected. These observations suggest that the main difference in structure between charged and uncharged tRNA resides in the release and exposure of the TpsiC loop for eventual binding to the ribosomes.

UI	MeSH Term	Description	Entries
D010649	Phenylalanine	An essential aromatic amino acid that is a precursor of MELANIN; DOPAMINE; noradrenalin (NOREPINEPHRINE), and THYROXINE.	Endorphenyl,L-Phenylalanine,Phenylalanine, L-Isomer,L-Isomer Phenylalanine,Phenylalanine, L Isomer
D004926	Escherichia coli	A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.	Alkalescens-Dispar Group,Bacillus coli,Bacterium coli,Bacterium coli commune,Diffusely Adherent Escherichia coli,E coli,EAggEC,Enteroaggregative Escherichia coli,Enterococcus coli,Diffusely Adherent E. coli,Enteroaggregative E. coli,Enteroinvasive E. coli,Enteroinvasive Escherichia coli
D012343	RNA, Transfer	The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.	Suppressor Transfer RNA,Transfer RNA,tRNA,RNA, Transfer, Suppressor,Transfer RNA, Suppressor,RNA, Suppressor Transfer
D046249	Transfer RNA Aminoacylation	The conversion of uncharged TRANSFER RNA to AMINO ACYL TRNA.	Aminoacylation, Transfer RNA,Transfer RNA Charging,tRNA Aminoacylation,tRNA Charging,Amino Acid Activation, Translational,Transfer RNA Acylation,Transfer RNA Amino Acylation,tRNA Acylation,tRNA Amino Acylation,Acylation, Transfer RNA,Acylation, tRNA,Acylations, Transfer RNA,Acylations, tRNA,Amino Acylation, tRNA,Aminoacylation, tRNA,Aminoacylations, Transfer RNA,Aminoacylations, tRNA,RNA Aminoacylations, Transfer,RNA Charging, Transfer,Transfer RNA Acylations,Transfer RNA Aminoacylations,Transfer RNA Chargings,tRNA Acylations,tRNA Aminoacylations,tRNA Chargings