-transparent.png){kind=logo}
The presence of voltage-dependent calcium channels has been suggested in mesangial cells by using calcium-sensitive fluorescent probes. However, direct electrophysiological evidence for voltage-dependent calcium channels has not yet been presented. In this study voltage-dependent calcium channels were studied in cultured rat mesangial cells. Whole-cell patch-clamp experiments were done with 50 mM Ba2+ as a charge carrier. Step depolarizing pulses from a holding potential of -50 mV produced an inward barium current at potentials more positive than -10 mV, and a peak current (10-45 pA) was obtained at a membrane potential of approximately +30 mV. The inward current was augmented by 100 nM Bay K 8644, attenuated by 1 microM nifedipine, and abolished by 50 microM Cd2+. These results indicate that the inward current is a barium current flowing through L-type calcium channels. This may be the first study that demonstrates the presence of L-type calcium channels in mesangial cells.
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
November 1991,
The American journal of physiology,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
November 1993,
The Journal of pharmacology and experimental therapeutics,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
September 1994,
Biochemical and biophysical research communications,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
March 1993,
Kidney international,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
July 1997,
The American journal of physiology,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
November 1985,
FEBS letters,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
November 2000,
The Journal of membrane biology,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
October 1989,
British journal of pharmacology,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
July 1994,
Kidney international,
M Nishio, and
H Tsukahara, and
M Hiraoka, and
M Sudo, and
S Kigoshi, and
I Muramatsu
September 1984,
Proceedings of the Royal Society of London. Series B, Biological sciences,
