-transparent.png){kind=logo}
Human immunodeficiency virus (HIV-I) reverse transcriptase was expressed in E. coli and purified to homogeneity (E. coli strain RRI (pRC-RT, pRK 248cIts)). We have investigated the substrate properties toward to DNA synthesis, catalyzed by this enzyme, of some nucleoside-5'-triphosphate analogues, previously studied in the same reactions, catalyzed by AMV and M-MLV reverse transcriptases. We have investigated substrate properties of new analogues of 2',3'-dideoxy-2',3'-didehydro- and 2',3'-dideoxytubercidin-5'-triphosphates. We have compared the relative efficiency of incorporation of different analogues tested in the DNA chain. It has been shown that expressed and purified HIV reverse transcriptase had the same specificity to analogues of 2'-deoxyribonucleoside-5'-triphosphates as was described for reverse transcriptases and natural HIV reverse transcriptase as well. These properties allow to apply the expressed HIV reverse transcriptase in different model systems.
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
July 1991,
Proceedings of the National Academy of Sciences of the United States of America,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
September 1993,
Nucleic acids research,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
July 1991,
Biochemistry,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
February 1996,
Biological chemistry Hoppe-Seyler,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
October 1989,
The Journal of biological chemistry,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
December 2010,
The Journal of biological chemistry,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
March 1992,
The EMBO journal,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
March 1995,
The Journal of biological chemistry,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
April 2002,
Biochemistry,
T A Rozovskaia, and
A A Belogurov, and
M A Lukin, and
D N Chernov, and
M K Kukhanova, and
R Sh Bibilashvili
September 1994,
Protein science : a publication of the Protein Society,
