BIOMAT Database Logo BIOMAT Database Logo

Inhibition of sporulation by cerulenin and its reversion by exogenous fatty acids in Saccharomyces cerevisiae. 1976

T Ohno, and J Awaya, and S Omura

Sporulation of Saccharomyces cerevisiae G2-2 was inhibited by the antibiotic cerulenin which is known to be a specific inhibitor of fatty acid and sterol synthesis. This inhibition was reversed by various fatty acids, especially by oleic acid (C(18:1)) and pentadecanoic acid (C(15:0)). Ergosterol showed only slight reversibility of this inhibition. When cerulenin was added to the sporulation medium later than 12 h after the start of incubation, the marked inhibition disappeared. When the fatty acid fraction extracted from the sporulated yeasts was added to the cells pretreated with cerulenin for more than 6 h, sporulation became evident 6 h after the fatty acid fraction addition. Therefore, sufficient synthesis of fatty acids required for sporulation was assumed to occur during the first 6 h in phase I of yeast sporulation.

UI MeSH Term Description Entries
D002569 Cerulenin An epoxydodecadienamide isolated from several species, including ACREMONIUM, Acrocylindrum, and Helicoceras. It inhibits the biosynthesis of several lipids by interfering with enzyme function. 2,3-Epoxy-4-oxo-7,10-dodecadienoylamide
D005227 Fatty Acids Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed) Aliphatic Acid,Esterified Fatty Acid,Fatty Acid,Fatty Acids, Esterified,Fatty Acids, Saturated,Saturated Fatty Acid,Aliphatic Acids,Acid, Aliphatic,Acid, Esterified Fatty,Acid, Saturated Fatty,Esterified Fatty Acids,Fatty Acid, Esterified,Fatty Acid, Saturated,Saturated Fatty Acids
D000935 Antifungal Agents Substances that destroy fungi by suppressing their ability to grow or reproduce. They differ from FUNGICIDES, INDUSTRIAL because they defend against fungi present in human or animal tissues. Anti-Fungal Agents,Antifungal Agent,Fungicides, Therapeutic,Antibiotics, Antifungal,Therapeutic Fungicides,Agent, Antifungal,Anti Fungal Agents,Antifungal Antibiotics
D012441 Saccharomyces cerevisiae A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement. Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D013172 Spores, Fungal Reproductive bodies produced by fungi. Conidia,Fungal Spores,Conidium,Fungal Spore,Spore, Fungal
D013997 Time Factors Elements of limited time intervals, contributing to particular results or situations. Time Series,Factor, Time,Time Factor

Related Publications

T Ohno, and J Awaya, and S Omura
Cerulenin inhibition of lipid synthesis and its reversal by exogenous fatty acids in Mycobacterium smegmatis ATCC 607.
February 1985, Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire,
T Ohno, and J Awaya, and S Omura
Erythromycin inhibition of sporulation in Saccharomyces cerevisiae.
August 1971, Experientia,
T Ohno, and J Awaya, and S Omura
Inhibition of sporulation by ethidium bromide and its reversal by fermentable sugars in Saccharomyces cerevisiae.
January 1974, Archives of microbiology,
T Ohno, and J Awaya, and S Omura
Reversal of cerulenin-induced inhibition of phospholipids and sterol synthesis by exogenous fatty acids/sterols in Epidermophyton floccosum.
September 1987, Biochimica et biophysica acta,
T Ohno, and J Awaya, and S Omura
The effect of cerulenin and exogenous fatty acids on triacylglycerol accumulation in an inositol-deficient yeast, Saccharomyces carlsbergensis.
May 1979, Biochimica et biophysica acta,
T Ohno, and J Awaya, and S Omura
Reduction of the acetyl coenzyme A carboxylase content of Saccharomyces cerevisiae by exogenous fatty acids.
December 1973, FEBS letters,
T Ohno, and J Awaya, and S Omura
Growth Inhibition by Amino Acids in Saccharomyces cerevisiae.
December 2020, Microorganisms,
T Ohno, and J Awaya, and S Omura
Promotion of sporulation by caffeine pretreatment in Saccharomyces cerevisiae. I. Metabolism of nucleic acids and protein during sporulation.
December 1975, Archives of microbiology,
T Ohno, and J Awaya, and S Omura
Inhibiton by sulfanilamide of sporulation in Saccharomyces cerevisiae.
June 1977, Canadian journal of microbiology,
T Ohno, and J Awaya, and S Omura
Amounts of free amino acids during early sporulation in Saccharomyces cerevisiae.
January 1996, Microbios,
Copied contents to your clipboard!