BIOMAT Database Logo BIOMAT Database Logo

Scanning electron microscopy of postoperative M-K and moist chamber-stored corneas. 1976

R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman

In the present study, using the in vivo test situation which all corneal donor material must meet, we performed penetrating keratoplastics utilizing corneal tissue stored for five days in either M-K medium or conventional moist chambers. Cat corneas stored for five days in M-K medium had a higher incidence of postoperative graft clarity along with an intact endothelial cell layer as determined by scanning electron microscopy than corneas stored in a conventional moist chamber. The endothelium of corneas immediately after moist chamber storage revealed minimal surface alteration which contrasted to the more severely stressed endothelium immediately after M-K storage. However, the M-K stored endothelium survived the trauma of penetrating keratoplasty much better than cells stored in moist chambers.

UI MeSH Term Description Entries
D002415 Cats The domestic cat, Felis catus, of the carnivore family FELIDAE, comprising over 30 different breeds. The domestic cat is descended primarily from the wild cat of Africa and extreme southwestern Asia. Though probably present in towns in Palestine as long ago as 7000 years, actual domestication occurred in Egypt about 4000 years ago. (From Walker's Mammals of the World, 6th ed, p801) Felis catus,Felis domesticus,Domestic Cats,Felis domestica,Felis sylvestris catus,Cat,Cat, Domestic,Cats, Domestic,Domestic Cat
D003315 Cornea The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed) Corneas
D004727 Endothelium A layer of epithelium that lines the heart, blood vessels (ENDOTHELIUM, VASCULAR), lymph vessels (ENDOTHELIUM, LYMPHATIC), and the serous cavities of the body. Endotheliums
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D012996 Solutions The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed) Solution
D014021 Tissue Preservation The process by which a tissue or aggregate of cells is kept alive outside of the organism from which it was derived (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism). Preservation, Tissue,Preservations, Tissue,Tissue Preservations
D014184 Transplantation, Homologous Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals. Transplantation, Allogeneic,Allogeneic Grafting,Allogeneic Transplantation,Allografting,Homografting,Homologous Transplantation,Grafting, Allogeneic
D016039 Corneal Transplantation Partial or total replacement of the CORNEA from one human or animal to another. Grafting, Corneal,Keratoplasty,Keratoplasty, Lamellar,Transplantation, Corneal,Cornea Transplantation,Transplantation, Cornea,Cornea Transplantations,Corneal Grafting,Corneal Graftings,Corneal Transplantations,Graftings, Corneal,Keratoplasties,Keratoplasties, Lamellar,Lamellar Keratoplasties,Lamellar Keratoplasty,Transplantations, Cornea,Transplantations, Corneal

Related Publications

R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
Ultrastructure of M-K and refrigerated moist chamber-stored corneas.
November 1974, Investigative ophthalmology,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
In vitro specular microscope perfusion of M-K- and moist chamber-stored human corneas.
August 1977, Investigative ophthalmology & visual science,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
Corneal transplantation with donor corneas stored in moist chamber and chondroitin sulfate-containing medium.
March 1993, Cornea,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
Ultrastructural changes in moist chamber corneas.
June 1963, Investigative ophthalmology,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
Dextran flux in M-K medium-stored human corneas.
August 1977, Investigative ophthalmology & visual science,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
HISTOCHEMICAL CHANGES IN ENDOTHELIUM OF CORNEAS STORED IN MOIST CHAMBERS.
December 1964, Archives of ophthalmology (Chicago, Ill. : 1960),
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
Spatial Resolution in Scanning Electron Microscopy and Scanning Transmission Electron Microscopy Without a Specimen Vacuum Chamber.
August 2016, Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
Scanning electron microscopy of moist bacterial strike-through of surgical materials.
February 1980, Surgery, gynecology & obstetrics,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
[Scanning electron microscopy findings in explanted posterior chamber lenses].
January 1989, Fortschritte der Ophthalmologie : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft,
R F Meyer, and B E McCarey, and J Valenti, and N Gravenstein, and H E Kaufman
[Corneal decompensation following Dannheim anterior chamber lens implantation--clinical aspects, scanning electron microscopy and transmission electron microscopy].
January 1988, Fortschritte der Ophthalmologie : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft,
Copied contents to your clipboard!