-transparent.png){kind=logo}
Nonopsonized Bordetella pertussis can bind to and become ingested by human monocytes. Previous studies demonstrated that mutant B. pertussis strains that lack fimbriae express a reduced adherence to monocytes. The present study was undertaken to investigate the involvement of the minor fimbrial subunit FimD in the adherence of B. pertussis to human monocytes using purified fimbriae, FimD, and strains with mutations in fimbrial genes. Flow cytometry demonstrated that purified B. pertussis fimbriae avidly bind to monocytes in a dose-dependent manner but bind less to neutrophils and hardly to lymphocytes; FimD-lacking fimbriae did not bind to monocytes. Purified fimbriae or FimD inhibited the binding of wild-type B. pertussis to monocytes in a dose-dependent manner and similarly inhibited the binding of a mutant defective in the major fimbrial subunits. It did not affect the binding of strains defective in FimD. These results prove that B. pertussis bind to human monocytes via the fimbrial minor subunit FimD.
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
October 1997,
Infection and immunity,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
July 1996,
Infection and immunity,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
January 1990,
FEMS microbiology letters,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
September 1986,
Journal of bacteriology,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
January 1988,
The Tokai journal of experimental and clinical medicine,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
January 1995,
Microbial pathogenesis,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
October 2014,
Expert review of vaccines,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
January 1996,
Journal of structural biology,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
September 1987,
Molecular microbiology,
W L Hazenbos, and
C A Geuijen, and
B M van den Berg, and
F R Mooi, and
R van Furth
August 1984,
Journal of medical microbiology,
