Biomaterial Database

The diameter of dentinal tubules in human coronal dentine after demineralization and air drying. A combined light microscopy and SEM study. 1995

J Arends, and I Stokroos, and W G Jongebloed, and J Ruben

Laboratory for Materia Technica, University of Groningen, The Netherlands.

The diameter of dentinal tubules after demineralization and/or air drying has been quantified using light microscopy and scanning electron microscopy (SEM). The tubule diameter was assessed at a distance of about 1.5 mm (range 1.0-2.0 mm) from the pulp. Tubule diameter was measured in the wet state, after 10-min and 12-hour air drying as well as after critical point drying (CO2) by SEM. The results show that drying effects on tubule diameter are small in sound tissue, but are sizeable in demineralized dentine. Comparing light microscopic (wet) and SEM observations show that the differences in tubule diameter are small for sound but substantial for demineralized dentine. An important result is that in the wet state the diameters of the tubules (being 1.3 +/- 0.2 microns in sound dentine) are 2.5 +/- 0.3, 2.2 +/- 0.3 and 1.7 +/- 0.2 microns after 1, 2 and 3 weeks of demineralization, respectively. The decreased tubule diameter with increasing demineralization may be important for permeability and transport phenomena in dentine caries and presumably in hypersensitivity.

UI	MeSH Term	Description	Entries
D008853	Microscopy	The use of instrumentation and techniques for visualizing material and details that cannot be seen by the unaided eye. It is usually done by enlarging images, transmitted by light or electron beams, with optical or magnetic lenses that magnify the entire image field. With scanning microscopy, images are generated by collecting output from the specimen in a point-by-point fashion, on a magnified scale, as it is scanned by a narrow beam of light or electrons, a laser, a conductive probe, or a topographical probe.	Compound Microscopy,Hand-Held Microscopy,Light Microscopy,Optical Microscopy,Simple Microscopy,Hand Held Microscopy,Microscopy, Compound,Microscopy, Hand-Held,Microscopy, Light,Microscopy, Optical,Microscopy, Simple
D008855	Microscopy, Electron, Scanning	Microscopy in which the object is examined directly by an electron beam scanning the specimen point-by-point. The image is constructed by detecting the products of specimen interactions that are projected above the plane of the sample, such as backscattered electrons. Although SCANNING TRANSMISSION ELECTRON MICROSCOPY also scans the specimen point by point with the electron beam, the image is constructed by detecting the electrons, or their interaction products that are transmitted through the sample plane, so that is a form of TRANSMISSION ELECTRON MICROSCOPY.	Scanning Electron Microscopy,Electron Scanning Microscopy,Electron Microscopies, Scanning,Electron Microscopy, Scanning,Electron Scanning Microscopies,Microscopies, Electron Scanning,Microscopies, Scanning Electron,Microscopy, Electron Scanning,Microscopy, Scanning Electron,Scanning Electron Microscopies,Scanning Microscopies, Electron,Scanning Microscopy, Electron
D003648	Decalcification Technique	Removal of minerals from bones during bone examination.	Decalcification Technic,Decalcification Technics,Decalcification Techniques,Technic, Decalcification,Technics, Decalcification,Technique, Decalcification,Techniques, Decalcification
D003731	Dental Caries	Localized destruction of the tooth surface initiated by decalcification of the enamel followed by enzymatic lysis of organic structures and leading to cavity formation. If left unchecked, the cavity may penetrate the enamel and dentin and reach the pulp.	Caries, Dental,Carious Lesions,Dental Cavities,Dental Cavity,Dental Decay,Dental White Spots,Carious Dentin,Decay, Dental,Dental White Spot,White Spot, Dental,White Spots, Dental,Carious Dentins,Carious Lesion,Cavities, Dental,Cavity, Dental,Dentin, Carious,Dentins, Carious,Lesion, Carious,Lesions, Carious,Spot, Dental White,Spots, Dental White
D003782	Dental Pulp	A richly vascularized and innervated connective tissue of mesodermal origin, contained in the central cavity of a tooth and delimited by the dentin, and having formative, nutritive, sensory, and protective functions. (Jablonski, Dictionary of Dentistry, 1992)	Dental Pulps,Pulp, Dental,Pulps, Dental
D003804	Dentin	The hard portion of the tooth surrounding the pulp, covered by enamel on the crown and cementum on the root, which is harder and denser than bone but softer than enamel, and is thus readily abraded when left unprotected. (From Jablonski, Dictionary of Dentistry, 1992)	Dentine,Dentines,Dentins
D003806	Dentin Permeability	The property of dentin that permits passage of light, heat, cold, and chemical substances. It does not include penetration by microorganisms.	Dentin Permeabilities,Permeabilities, Dentin,Permeability, Dentin
D003807	Dentin Sensitivity	Pain associated with exposed DENTIN surfaces of the teeth.	Dentin Hypersensitivity,Dentine Hypersensitivity,Dentine Sensitivity,Tooth Sensitivity,Dentin Hypersensitivities,Dentin Sensitivities,Dentine Hypersensitivities,Dentine Sensitivities,Hypersensitivities, Dentin,Hypersensitivities, Dentine,Hypersensitivity, Dentin,Hypersensitivity, Dentine,Sensitivities, Dentin,Sensitivities, Dentine,Sensitivities, Tooth,Sensitivity, Dentin,Sensitivity, Dentine,Sensitivity, Tooth,Tooth Sensitivities
D003890	Desiccation	Removal of moisture from a substance (chemical, food, tissue, etc.).	Dessication
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man