BIOMAT Database Logo BIOMAT Database Logo

Isolation of a cDNA for a growth factor of vascular endothelial cells from human lung cancer cells: its identity with insulin-like growth factor II. 1995

K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo.

We have found growth-promoting activity for vascular endothelial cells in the conditioned medium of a human lung cancer cell line, T3M-11. Purification and characterization of the growth-promoting activity have been carried out using ammonium sulfate precipitation and gel-exclusion chromatography. The activity migrated as a single peak just after ribonuclease. It did not bind to a heparin affinity column. These results suggest that the activity is not a heparin-binding growth factor (including fibroblast growth factors) or a vascular endothelial growth factor. To identify the molecule exhibiting the growth-promoting activity, a cDNA encoding the growth factor was isolated through functional expression cloning in COS-1 cells from a cDNA library prepared from T3M-11 cells. The nucleotide sequence encoded by the cDNA proved to be identical with that of insulin-like growth factor II.

UI MeSH Term Description Entries
D007335 Insulin-Like Growth Factor II A well-characterized neutral peptide believed to be secreted by the LIVER and to circulate in the BLOOD. It has growth-regulating, insulin-like and mitogenic activities. The growth factor has a major, but not absolute, dependence on SOMATOTROPIN. It is believed to be a major fetal growth factor in contrast to INSULIN-LIKE GROWTH FACTOR I, which is a major growth factor in adults. IGF-II,Multiplication-Stimulating Activity,Somatomedin MSA,IGF-2,Insulin Like Growth Factor II,Insulin-Like Somatomedin Peptide II,Multiplication-Stimulating Factor,Somatomedin A,Factor, Multiplication-Stimulating,Insulin Like Somatomedin Peptide II,Multiplication Stimulating Activity,Multiplication Stimulating Factor
D008175 Lung Neoplasms Tumors or cancer of the LUNG. Cancer of Lung,Lung Cancer,Pulmonary Cancer,Pulmonary Neoplasms,Cancer of the Lung,Neoplasms, Lung,Neoplasms, Pulmonary,Cancer, Lung,Cancer, Pulmonary,Cancers, Lung,Cancers, Pulmonary,Lung Cancers,Lung Neoplasm,Neoplasm, Lung,Neoplasm, Pulmonary,Pulmonary Cancers,Pulmonary Neoplasm
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D002455 Cell Division The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION. M Phase,Cell Division Phase,Cell Divisions,Division Phase, Cell,Division, Cell,Divisions, Cell,M Phases,Phase, Cell Division,Phase, M,Phases, M
D004730 Endothelium, Vascular Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components. Capillary Endothelium,Vascular Endothelium,Capillary Endotheliums,Endothelium, Capillary,Endotheliums, Capillary,Endotheliums, Vascular,Vascular Endotheliums
D006133 Growth Substances Signal molecules that are involved in the control of cell growth and differentiation. Mitogens, Endogenous,Endogenous Mitogens
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D001011 Aorta The main trunk of the systemic arteries. Aortas
D001483 Base Sequence The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence. DNA Sequence,Nucleotide Sequence,RNA Sequence,DNA Sequences,Base Sequences,Nucleotide Sequences,RNA Sequences,Sequence, Base,Sequence, DNA,Sequence, Nucleotide,Sequence, RNA,Sequences, Base,Sequences, DNA,Sequences, Nucleotide,Sequences, RNA

Related Publications

K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Isolation of an insulin-like growth factor II cDNA with a unique 5' untranslated region from human placenta.
March 1988, Proceedings of the National Academy of Sciences of the United States of America,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Isolation of a cDNA clone encoding rat insulin-like growth factor-II precursor.
January 1984, Nature,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Expression of a human insulin-like growth factor II cDNA in NIH-3T3 cells.
June 1990, Biochemical and biophysical research communications,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Comparative studies on insulin-like growth factor II and insulin processing by vascular endothelial cells.
October 1986, Diabetes,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Isolation and characterization of insulin-like growth factor-II from human bone.
March 1988, Biochemical and biophysical research communications,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Influence of vascular endothelial growth factor on the expression of insulin-like growth factor-II, insulin-like growth factor binding protein-2 and 5 in human luteinized granulosa cells.
November 2012, Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Intrinsic bioactivity of insulin-like growth factor-binding proteins from vascular endothelial cells.
December 1990, Endocrinology,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Isolation of the human insulin-like growth factor genes: insulin-like growth factor II and insulin genes are contiguous.
October 1985, Proceedings of the National Academy of Sciences of the United States of America,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
Regulation of vascular endothelial growth factor expression in human colon cancer by insulin-like growth factor-I.
September 1998, Cancer research,
K Hagiwara, and T Kobayashi, and M Tobita, and N Kikyo, and Y Yazaki, and T Okabe
[Cloning of human vascular endothelial growth factor cDNA and its expression in COS-7 cells]
August 2002, Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences,
Copied contents to your clipboard!