BIOMAT Database Logo BIOMAT Database Logo

[Detection of human papillomavirus type 16, 18 and 33 DNA in stage I (pT1N0M0) squamous cell carcinoma of the lung by polymerase chain reaction]. 1995

M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Department of Thoracic Surgery, Tohoku University, Sendai, Japan.

Human papillomavirus (HPV) has been considered one of the important factors of malignant change. To investigate the occurrence of HPV DNA in primary squamous cell carcinoma of the lung, we tried to detect HPV DNA by polymerase chain reaction (PCR). Formalin-fixed paraffin-embedded tissue specimens from 8 cases of stage I (pT1N0M0) squamous cell carcinoma of the lung were examined for the presence of human papillomavirus DNA (type 16, 18 and 33) by polymerase chain reaction. DNA was extracted from double 5 microns sections of each specimen by deparaffinization and proteinase K digestion. Using the primers described previously by Shimada et al, target DNA sequences were selectively amplified through 40 cycles of 94 degrees C for 1 min, 55 degrees C for 2 min, and 72 degrees C for 2 min. When stained band was unclear, second PCR was conducted using product of first PCR as template of second PCR. Human papillomavirus type 18 was present in 1 case (12.5%) in contrast that type 16 and 33 were not detected in these cases.

UI MeSH Term Description Entries
D008175 Lung Neoplasms Tumors or cancer of the LUNG. Cancer of Lung,Lung Cancer,Pulmonary Cancer,Pulmonary Neoplasms,Cancer of the Lung,Neoplasms, Lung,Neoplasms, Pulmonary,Cancer, Lung,Cancer, Pulmonary,Cancers, Lung,Cancers, Pulmonary,Lung Cancers,Lung Neoplasm,Neoplasm, Lung,Neoplasm, Pulmonary,Pulmonary Cancers,Pulmonary Neoplasm
D008297 Male Males
D008875 Middle Aged An adult aged 45 - 64 years. Middle Age
D002294 Carcinoma, Squamous Cell A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed) Carcinoma, Epidermoid,Carcinoma, Planocellular,Carcinoma, Squamous,Squamous Cell Carcinoma,Carcinomas, Epidermoid,Carcinomas, Planocellular,Carcinomas, Squamous,Carcinomas, Squamous Cell,Epidermoid Carcinoma,Epidermoid Carcinomas,Planocellular Carcinoma,Planocellular Carcinomas,Squamous Carcinoma,Squamous Carcinomas,Squamous Cell Carcinomas
D004279 DNA, Viral Deoxyribonucleic acid that makes up the genetic material of viruses. Viral DNA
D005260 Female Females
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D000368 Aged A person 65 years of age or older. For a person older than 79 years, AGED, 80 AND OVER is available. Elderly
D014412 Tumor Virus Infections Infections produced by oncogenic viruses. The infections caused by DNA viruses are less numerous but more diverse than those caused by the RNA oncogenic viruses. Fibroma, Shope,Papilloma, Shope,Infections, Tumor Virus,Infection, Tumor Virus,Shope Fibroma,Shope Papilloma,Tumor Virus Infection

Related Publications

M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Detection of human papillomavirus DNA in laryngeal squamous cell carcinoma by polymerase chain reaction.
May 1996, European journal of cancer (Oxford, England : 1990),
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
[Detection of human papillomavirus types 16, 18 and 33 in exfoliated cervical cells by polymerase chain reaction].
December 1991, Nihon Sanka Fujinka Gakkai zasshi,
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Detection of human papillomavirus types 16, 18 DNA related sequences in bronchogenic carcinoma by polymerase chain reaction.
August 1995, Chinese medical journal,
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Detection of human papillomavirus type 16 in oropharyngeal squamous cell carcinoma using droplet digital polymerase chain reaction.
May 2016, Cancer,
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
[Detection of human papillomavirus DNA in squamous cell carcinomas of the lung by multiple polymerase chain reaction].
October 1993, Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases,
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Detection of human papillomavirus DNA in laryngeal squamous cell carcinomas by polymerase chain reaction.
June 1990, The Laryngoscope,
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Polymerase chain reaction protocols for the detection of DNA from mucosal human papillomavirus types -6, -11, -16, -18, -31 and -33.
July 1997, Journal of virological methods,
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Detection of human papillomavirus in squamous cell carcinomas of the lung by polymerase chain reaction.
February 1998, American journal of respiratory cell and molecular biology,
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Detection of human papillomavirus DNA in primary lung carcinoma by nested polymerase chain reaction.
December 1995, Cellular and molecular biology (Noisy-le-Grand, France),
M Sagawa, and Y Saito, and C Endo, and M Sato, and K Usuda, and K Kanma, and S Takahashi, and E Chin, and A Sakurada, and K Aikawa
Detection of type 16 human papillomavirus DNA in formalin-fixed invasive squamous cells from laryngeal cancers by polymerase chain reaction.
January 1998, Memorias do Instituto Oswaldo Cruz,
Copied contents to your clipboard!