-transparent.png){kind=logo}
We have isolated a segment of DNA from the eukaryote Saccharomyces cerevisiae (baker's yeast) as a viable molecular hybrid of bacteriophage lambda DNA which, when integrated into the chromosome of an E. coli histidine auxotroph, allows this bacterium to grow in the absence of histidine. The nonrevertable, histidine auxotroph lacks the enzymatic activity of imidazole glycerol phosphate (IGP) dehydratase (EC 4.2.1.19). From genetic experiments, we conclude that expression of the segment of yeast DNA results in the production of a diffusible substance and that transcription necessary for the complementation is most likely initiated from the segment of eukaryotic DNA.
Related Publications
K Struhl, and
J R Cameron, and
R W Davis
August 1998,
Nucleic acids research,
K Struhl, and
J R Cameron, and
R W Davis
February 1977,
Proceedings of the National Academy of Sciences of the United States of America,
K Struhl, and
J R Cameron, and
R W Davis
December 1979,
Genetika,
K Struhl, and
J R Cameron, and
R W Davis
May 1974,
Proceedings of the National Academy of Sciences of the United States of America,
K Struhl, and
J R Cameron, and
R W Davis
October 1989,
The Journal of biological chemistry,
K Struhl, and
J R Cameron, and
R W Davis
January 1996,
Methods in enzymology,
K Struhl, and
J R Cameron, and
R W Davis
May 2004,
Sheng wu gong cheng xue bao = Chinese journal of biotechnology,
K Struhl, and
J R Cameron, and
R W Davis
January 1986,
Biotechnology advances,
K Struhl, and
J R Cameron, and
R W Davis
March 1998,
Protein expression and purification,
K Struhl, and
J R Cameron, and
R W Davis
August 2006,
Science (New York, N.Y.),
