Biomaterial Database

RNA polymerase III transcription in the yeast Saccharomyces cerevisiae. 1994

S Buratowski

Whitehead Institute for Biomedical Research, Cambridge, MA 02142.

The yeast RNA polymerase III system is probably the best-characterized eukaryotic transcription system. Nearly all of the components have been identified and the genes for them cloned. Many of the interactions within initiation complexes are coming to light. Considering the many parallels between Pol III transcription and the other polymerase systems, findings in the Pol III system can act as predictions for Pol II and Pol I transcription. Despite the many advances made in the study of transcription by RNA polymerase III, many important questions remain to be answered. It is unclear what are the functions of individual TFIIIC, TFIIIB and polymerase subunits. Why are so many proteins required? Another extremely important mystery is the mechanism by which the factors assemble. What is the molecular mechanism for TFIIIC recruiting TFIIIB, and how does TFIIIB recruit polymerase? These and many other problems will eventually be solved as researchers apply the biochemical and genetic techniques available in the yeast system.

UI	MeSH Term	Description	Entries
D012320	RNA Polymerase III	A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure where it transcribes DNA into RNA. It has specific requirements for cations and salt and has shown an intermediate sensitivity to alpha-amanitin in comparison to RNA polymerase I and II.	DNA-Dependent RNA Polymerase III,RNA Polymerase C,DNA Dependent RNA Polymerase III,Polymerase C, RNA,Polymerase III, RNA
D012441	Saccharomyces cerevisiae	A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.	Baker's Yeast,Brewer's Yeast,Candida robusta,S. cerevisiae,Saccharomyces capensis,Saccharomyces italicus,Saccharomyces oviformis,Saccharomyces uvarum var. melibiosus,Yeast, Baker's,Yeast, Brewer's,Baker Yeast,S cerevisiae,Baker's Yeasts,Yeast, Baker
D014157	Transcription Factors	Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.	Transcription Factor,Factor, Transcription,Factors, Transcription
D014158	Transcription, Genetic	The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.	Genetic Transcription