Biomaterial Database

[Cytochemical evidence for endogenous hydrogen peroxide generation in the neutrophils from human blood smears]. 1994

V V Rogovin, and P G But

We present more accurate evidence for generation of endogenous hydrogen peroxide as a result of peroxidase-endogenous hydrogen peroxide system activity in the neutrophils (Rogovin et al., 1978). The normal cytochemical reaction of the peroxidase-endogenous hydrogen peroxide system in the neutrophil peroxidasosomes after elimination of water-dissolved H2O2 by KMnO4 indicates the neutrophils on smears as a source of H2O2, rather than alcohol-formaldehyde. This is confirmed by the cytochemical reaction of peroxidase-endogenous H2O2 reaction in the neutrophils on smears prepared without fixation. The peroxidase-endogenous H2O2 system in the neutrophils on blood smears acts at pH other than peroxidase: 7.7-5.5 vs. 7.7-3.4 (basic pH range was not examined). This difference appear to be due specific features of the enzyme complexes ultimately generating H2O2.

UI	MeSH Term	Description	Entries
D008722	Methods	A series of steps taken in order to conduct research.	Techniques,Methodological Studies,Methodological Study,Procedures,Studies, Methodological,Study, Methodological,Method,Procedure,Technique
D009195	Peroxidase	A hemeprotein from leukocytes. Deficiency of this enzyme leads to a hereditary disorder coupled with disseminated moniliasis. It catalyzes the conversion of a donor and peroxide to an oxidized donor and water. EC 1.11.1.7.	Myeloperoxidase,Hemi-Myeloperoxidase,Hemi Myeloperoxidase
D009504	Neutrophils	Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.	LE Cells,Leukocytes, Polymorphonuclear,Polymorphonuclear Leukocytes,Polymorphonuclear Neutrophils,Neutrophil Band Cells,Band Cell, Neutrophil,Cell, LE,LE Cell,Leukocyte, Polymorphonuclear,Neutrophil,Neutrophil Band Cell,Neutrophil, Polymorphonuclear,Polymorphonuclear Leukocyte,Polymorphonuclear Neutrophil
D006651	Histocytochemistry	Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.	Cytochemistry
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006861	Hydrogen Peroxide	A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.	Hydrogen Peroxide (H2O2),Hydroperoxide,Oxydol,Perhydrol,Superoxol,Peroxide, Hydrogen
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations