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Homocystinuria is an inborn error of methionine metabolism that is characterized by the premature development of arteriosclerosis. As one of the major factors in the pathogenesis of arteriosclerosis, modification of low-density lipoprotein (LDL) has received widespread attention by many investigators. In this study, to elucidate the relationship between elevated homocysteine levels and premature arteriosclerosis, we investigated the role of homocysteine in the iron-catalyzed oxidative modification of LDL. When LDL isolated from a healthy subject was incubated with homocysteine and ferric ion, a gradual decrease of polyunsaturated fatty acids (PUFA), formation of thiobarbituric acid-reactive substances (TBARS) and fluorescent substances, and the fragmentation of apoprotein B (apoB) were observed. The extent of oxidative modification was dependent on the concentration of homocysteine. Modification of LDL was suppressed until the remaining alpha-tocopherol concentration reached a critical level. When the alpha-tocopherol content of LDL was increased by 2.6-fold, both the formation of TBARS and the fragmentation of apoB were suppressed. These results suggest that homocysteine might promote iron-catalyzed oxidation of LDL and imply its role for the development of premature arteriosclerosis.
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
March 1991,
Proceedings of the National Academy of Sciences of the United States of America,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
January 1988,
Basic life sciences,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
June 2002,
Chemical research in toxicology,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
December 1996,
The Journal of biological chemistry,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
January 1990,
The Biochemical journal,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
October 2000,
Journal of lipid research,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
August 1991,
Xenobiotica; the fate of foreign compounds in biological systems,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
June 1994,
The Journal of biological chemistry,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
August 1983,
Journal of lipid research,
K Hirano, and
T Ogihara, and
M Miki, and
H Yasuda, and
H Tamai, and
N Kawamura, and
M Mino
June 1984,
Proceedings of the National Academy of Sciences of the United States of America,
