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Highly purified MOPC-21 IgG1, MOPC-173 IgG2a, MOPC-195 IgG2b, MOPC-104 E IgM, and MOPC-315 IgA paraproteins, heterogeneous mouse IgG, Fab and Fc fragments of heterogeneous IgG were prepared and coupled to Sepharose beads. These beads were then used as artificial substrates to test the specificity of fluorescent antisera against mouse immunoglobulins by microflurometry. By comparing the visual evaluation of strained plasma cells and measurements on beads, the highest permissible percentage (FITC) and 6% for a tetramethyl rhodamine iso thiocyanate (TRITC) conjugate. By application of these criteria, 1 out of 7 tested commercial antisera and 6 out of 8 conjugates prepared in this laboratory proved to be satisfactory. The most common impurities were anti-light chain antibodies, as revealed by their reaction with Fab. With the bead system, a good impression of the specificity of an antiserum can be obtained. It gives, however, only approximate information on whether the conjugate will cause a high background staining in the biological specimen.
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
June 1975,
Annals of the New York Academy of Sciences,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
November 1974,
Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
March 1976,
Zeitschrift fur Immunitatsforschung, experimentelle und klinische Immunologie,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
June 1975,
Annals of the New York Academy of Sciences,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
January 1975,
Annales de la Societe belge de medecine tropicale,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
March 1974,
Journal of immunological methods,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
April 1975,
Experimental parasitology,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
August 1974,
Journal of immunological methods,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
June 1975,
Experimental parasitology,
F J Bloemmen, and
J Rádl, and
J J Haaijman, and
P Van den Berg, and
H R Schuit, and
W Hijmans
November 1978,
Experimental parasitology,
