-transparent.png){kind=logo}
Endothelin (ET) is a 21-residue potent vasoconstrictive peptide produced by vascular endothelial cells and formed from its precursor, big endothelin (big ET), by endothelin-converting enzyme (ECE). This paper describes the cloning and functional expression of a cDNA encoding a human ECE from human umbilical vein endothelial cells (HUVEC). Human ECE consists of 758 amino acid residues and has high homology to rat and bovine ECE. Immunoblot analysis using a monoclonal antibody risen against rat lung ECE showed the presence of immunoreactive protein in membrane fraction prepared from both HUVEC and COS-1 cells transfected with human ECE cDNA. Both COS-1 cells expressing human ECE and its membrane fraction converted big ET-1 most efficiently among big ETs.
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
September 1994,
Biochemical and biophysical research communications,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
July 1994,
The Journal of biological chemistry,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
July 1991,
Biochemical and biophysical research communications,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
October 2006,
DNA sequence : the journal of DNA sequencing and mapping,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
August 1991,
FEBS letters,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
September 1999,
Biochimica et biophysica acta,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
February 1998,
Biochemistry. Biokhimiia,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
November 1997,
The Journal of clinical endocrinology and metabolism,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
June 1995,
Biochemical and biophysical research communications,
K Shimada, and
Y Matsushita, and
K Wakabayashi, and
M Takahashi, and
A Matsubara, and
Y Iijima, and
K Tanzawa
March 1995,
Biochemical and biophysical research communications,
