Biomaterial Database

[Interaction of divalent copper ions with native DNA and its monomers]. 1994

V A Sorokin

The complex formation of Cu2+ ions with the 2'-deoxynucleotide-5'-phosphates of bases in water solution by the UV-spectroscopy method has been established. It was obtained that the N7-atom in dGMP and N3-atom in dTMP are coordinated with copper (Cu) in complexes. The deoxyribonucleotide C can not subtract with the copper (Cu). The constants (K) of Cu-bases binding have been obtained. The K-value for purines is greater than for pyrimidines independent of the ribose structure. The ions Cu2+ at formation of complexes with separate nucleotides or natural DNA can cause the conformational transitions in these molecules. Thus the ions Cu2+ interact as with N7 and O6 G, as with N3 and O2 C. It is agreed with model, in which guanosine passes from anti to sin-conformation. The interactions of Cu-ions with G, C and A base pairs in single stranded DNA have been registered.

UI	MeSH Term	Description	Entries
D009690	Nucleic Acid Conformation	The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.	DNA Conformation,RNA Conformation,Conformation, DNA,Conformation, Nucleic Acid,Conformation, RNA,Conformations, DNA,Conformations, Nucleic Acid,Conformations, RNA,DNA Conformations,Nucleic Acid Conformations,RNA Conformations
D002413	Cations, Divalent	Positively charged atoms, radicals or groups of atoms with a valence of plus 2, which travel to the cathode or negative pole during electrolysis.	Divalent Cations
D003300	Copper	A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.	Copper-63,Copper 63
D003854	Deoxyribonucleotides	A purine or pyrimidine base bonded to a DEOXYRIBOSE containing a bond to a phosphate group.	Deoxyribonucleotide
D004247	DNA	A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).	DNA, Double-Stranded,Deoxyribonucleic Acid,ds-DNA,DNA, Double Stranded,Double-Stranded DNA,ds DNA
D013056	Spectrophotometry, Ultraviolet	Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)	Ultraviolet Spectrophotometry