Biomaterial Database

Kinetic characterization of sporulation in Streptomyces albidoflavus SMF301 during submerged culture. 1994

Y T Rho, and K J Lee

Department of Microbiology, Seoul National University, Korea.

We report the first quantitative analysis of the relationship between environmental changes and sporulation of a streptomycete, Streptomyces albidoflavus SMF301, in submerged culture. A chemically defined medium was constructed for sporulation, over 10(9) spores ml-1 being formed in the submerged batch culture. Kinetic parameters calculated from batch and chemostat cultures showed that specific submerged spore formation rate (qspo) was inversely related to the specific mycelial growth rate (mu). The optimum growth rate for submerged spore formation was 0.05 h-1, when the maximum value of qspo was 1.0 x 10(6) spores g-1 h-1. The turnover rate of biomass at maximum growth yield was 0.029 h-1, when 5.6 x 10(6) spores were formed from 1 g mycelium. The present quantitative analysis of submerged spore formation using a controlled system opens the way for biochemical and molecular biological studies related to the morphological differentiation of Streptomyces spp.

UI	MeSH Term	Description	Entries
D007700	Kinetics	The rate dynamics in chemical or physical systems.
D005947	Glucose	A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.	Dextrose,Anhydrous Dextrose,D-Glucose,Glucose Monohydrate,Glucose, (DL)-Isomer,Glucose, (alpha-D)-Isomer,Glucose, (beta-D)-Isomer,D Glucose,Dextrose, Anhydrous,Monohydrate, Glucose
D000644	Quaternary Ammonium Compounds	Derivatives of ammonium compounds, NH4+ Y-, in which all four of the hydrogens bonded to nitrogen have been replaced with hydrocarbyl groups. These are distinguished from IMINES which are RN	Quaternary Ammonium Compound,Ammonium Compound, Quaternary,Ammonium Compounds, Quaternary,Compound, Quaternary Ammonium
D001431	Bacteriological Techniques	Techniques used in studying bacteria.	Bacteriologic Technic,Bacteriologic Technics,Bacteriologic Techniques,Bacteriological Technique,Technic, Bacteriological,Technics, Bacteriological,Technique, Bacteriological,Techniques, Bacteriological,Bacteriologic Technique,Bacteriological Technic,Bacteriological Technics,Technic, Bacteriologic,Technics, Bacteriologic,Technique, Bacteriologic,Techniques, Bacteriologic
D013171	Spores, Bacterial	Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.	Bacterial Spores,Bacterial Spore,Spore, Bacterial
D013302	Streptomyces	A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.