Biomaterial Database

Decreased immunoreactivity of platelet-derived growth factor B chain-like peptide after axotomy in the dorsal motor nucleus of the vagus nerve. 1994

E Yamada, and F Hazama

Department of Pathology, Shiga University of Medical Science, Otsu, Japan.

Platelet-derived growth factor-B chain (PDGF-B) and B chain-specific beta receptor (PDGF-R) were investigated immunohistochemically in the dorsal motor nucleus of the vagus nerve and hypoglossal nucleus after axotomy using antibodies raised against synthetic polypeptides. PDGF-B and PDGF-R immunoreactivity were observed in nerve cell bodies contralateral to the axotomized nerve in both vagal (degenerative) and hypoglossal (regenerative) nuclei. The immunoreactivity for PDGF-B antibody persisted until day 28 after axotomy in the hypoglossal neurons, while that in many neurons in the vagal nucleus diminished after day 3. In the severed vagal nucleus some of the axotomized neurons showed no immunoreactivity for PDGF-B chain, and these changes preceded the decrease in neuronal numbers in the vagal nucleus. The immunoreactivity for PDGF-R antibody showed no marked change in either the vagal or hypoglossal nucleus until day 28 after axotomy. These findings suggest that the decrease in PDGF-B immunoreactivity is not due to a non-specific depletion of cytoplasmic protein in the severed vagal neurons. PDGF, taken up by the nucleus and bound to chromatin, has been reported to exert direct effects on the enhancement of transcription and synthesis of RNA. The decrease in level of PDGF-B chain in the vagal neurons seems to cause the reduction of RNA and protein synthesis, resulting in neuronal degeneration.

UI	MeSH Term	Description	Entries
D007002	Hypoglossal Nerve	The 12th cranial nerve. The hypoglossal nerve originates in the hypoglossal nucleus of the medulla and supplies motor innervation to all of the muscles of the tongue except the palatoglossus (which is supplied by the vagus). This nerve also contains proprioceptive afferents from the tongue muscles.	Cranial Nerve XII,Twelfth Cranial Nerve,Nerve XII,Nervus Hypoglossus,Cranial Nerve XIIs,Cranial Nerve, Twelfth,Cranial Nerves, Twelfth,Hypoglossal Nerves,Hypoglossus, Nervus,Nerve XII, Cranial,Nerve XIIs,Nerve XIIs, Cranial,Nerve, Hypoglossal,Nerve, Twelfth Cranial,Nerves, Hypoglossal,Nerves, Twelfth Cranial,Twelfth Cranial Nerves,XII, Nerve,XIIs, Nerve
D009046	Motor Neurons	Neurons which activate MUSCLE CELLS.	Neurons, Motor,Alpha Motorneurons,Motoneurons,Motor Neurons, Alpha,Neurons, Alpha Motor,Alpha Motor Neuron,Alpha Motor Neurons,Alpha Motorneuron,Motoneuron,Motor Neuron,Motor Neuron, Alpha,Motorneuron, Alpha,Motorneurons, Alpha,Neuron, Alpha Motor,Neuron, Motor
D010982	Platelet-Derived Growth Factor	Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.	Platelet Derived Growth Factor,Factor, Platelet-Derived Growth,Growth Factor, Platelet-Derived
D011518	Proto-Oncogene Proteins	Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.	Cellular Proto-Oncogene Proteins,c-onc Proteins,Proto Oncogene Proteins, Cellular,Proto-Oncogene Products, Cellular,Cellular Proto Oncogene Proteins,Cellular Proto-Oncogene Products,Proto Oncogene Products, Cellular,Proto Oncogene Proteins,Proto-Oncogene Proteins, Cellular,c onc Proteins
D003714	Denervation	The resection or removal of the nerve to an organ or part.	Laser Neurectomy,Neurectomy,Peripheral Neurectomy,Radiofrequency Neurotomy,Denervations,Laser Neurectomies,Neurectomies,Neurectomies, Laser,Neurectomies, Peripheral,Neurectomy, Laser,Neurectomy, Peripheral,Neurotomies, Radiofrequency,Neurotomy, Radiofrequency,Peripheral Neurectomies,Radiofrequency Neurotomies
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D001369	Axons	Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.	Axon
D014630	Vagus Nerve	The 10th cranial nerve. The vagus is a mixed nerve which contains somatic afferents (from skin in back of the ear and the external auditory meatus), visceral afferents (from the pharynx, larynx, thorax, and abdomen), parasympathetic efferents (to the thorax and abdomen), and efferents to striated muscle (of the larynx and pharynx).	Cranial Nerve X,Pneumogastric Nerve,Tenth Cranial Nerve,Nerve X,Nervus Vagus,Cranial Nerve, Tenth,Cranial Nerves, Tenth,Nerve X, Cranial,Nerve Xs,Nerve, Pneumogastric,Nerve, Tenth Cranial,Nerve, Vagus,Nerves, Pneumogastric,Nerves, Tenth Cranial,Nerves, Vagus,Pneumogastric Nerves,Tenth Cranial Nerves,Vagus Nerves,Vagus, Nervus
D017479	Receptors, Platelet-Derived Growth Factor	Specific receptors on cell membranes that react with PLATELET-DERIVED GROWTH FACTOR, its analogs, or antagonists. The alpha PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR ALPHA) and the beta PDGF receptor (RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA) are the two principle types of PDGF receptors. Activation of the protein-tyrosine kinase activity of the receptors occurs by ligand-induced dimerization or heterodimerization of PDGF receptor types.	PDGF Receptors,Platelet-Derived Growth Factor Receptors,Receptors, PDGF,PDGF Receptor,Platelet-Derived Growth Factor Receptor,Platelet Derived Growth Factor Receptor,Platelet Derived Growth Factor Receptors,Receptor, PDGF,Receptors, Platelet Derived Growth Factor
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus