Hydroxyapatite ceramics for continuous delivery of heparin. 1994

L Abrams, and P K Bajpai
University of Dayton, Department of Biology, OH 45469-2320.

The goal of this investigation is to develop a ceramic system for delivering therapeutic amounts of heparin continuously from subcutaneous implants. Hydroxyapatite (HA) ceramic reservoirs were used for the continuous delivery of heparin in both in vitro and in vivo environments. Each reservoir was loaded with heparin. The open end was then sealed with a silastic medical adhesive and sterilized by ethylene oxide. A total of 15 capsules were used for conducting the in vitro experiments. Each capsule (control, sham, 1400, 2800, 4200 units heparin) was suspended in a vial containing 100 ml phosphate buffered saline (pH 7.4) at 37 degrees C. A one ml aliquot was withdrawn from each serum bottle at fixed intervals for three weeks, and assayed for heparin by the Sigma Heparin Clotting Assay. The largest amount of heparin was released from the capsule containing 4200 units of heparin and the smallest amount was released from the capsule containing 1400 units of heparin. Thirty Holtzman rats (140 +/- 15 g.) were used in the in vivo experiment. The rats were equally distributed into five groups (control, sham, 1400, 2800, and 4200 units of heparin). Animals in four of the five groups were implanted with HA capsules containing various amounts of heparin. Capillary tube clotting times were observed at regular intervals. Six days following implantation, the clotting times of the blood obtained from rats implanted with heparin-containing capsules was significantly higher (6.3 +/- 0.07 minutes) than the clotting times of blood obtained from shams and controls (4.3 +/- 0.03 minutes).(ABSTRACT TRUNCATED AT 250 WORDS)

UI MeSH Term Description Entries
D002516 Ceramics Products made by baking or firing nonmetallic minerals (clay and similar materials). In making dental restorations or parts of restorations the material is fused porcelain. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed & Boucher's Clinical Dental Terminology, 4th ed) Ceramic
D004343 Drug Implants Small containers or pellets of a solid drug implanted in the body to achieve sustained release of the drug. Drug Implant,Drug Pellet,Pellets, Drug,Drug Pellets,Implant, Drug,Implants, Drug,Pellet, Drug
D006493 Heparin A highly acidic mucopolysaccharide formed of equal parts of sulfated D-glucosamine and D-glucuronic acid with sulfaminic bridges. The molecular weight ranges from six to twenty thousand. Heparin occurs in and is obtained from liver, lung, mast cells, etc., of vertebrates. Its function is unknown, but it is used to prevent blood clotting in vivo and vitro, in the form of many different salts. Heparinic Acid,alpha-Heparin,Heparin Sodium,Liquaemin,Sodium Heparin,Unfractionated Heparin,Heparin, Sodium,Heparin, Unfractionated,alpha Heparin
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D051381 Rats The common name for the genus Rattus. Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D017886 Durapatite The mineral component of bones and teeth; it has been used therapeutically as a prosthetic aid and in the prevention and treatment of osteoporosis. Calcium Hydroxyapatite,Hydroxyapatite,Hydroxylapatite,Alveograf,Calcitite,Interpore-200,Interpore-500,Osprovit,Ossein-Hydroxyapatite Compound,Ossopan,Osteogen,Periograf,Hydroxyapatite, Calcium,Interpore 200,Interpore 500,Interpore200,Interpore500,Ossein Hydroxyapatite Compound

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