-transparent.png){kind=logo}
The up-regulation of the cyclic AMP system and enhanced phosphorylation of protein substrates after either sudden interruption of chronic opioid treatment or antagonist administration has been proposed to account for the various behavioral responses observed during withdrawal. Using in situ hybridization histochemistry, we show here for the first time that type VIII adenylyl cyclase mRNA is selectively increased, as early as 12 h after morphine treatment in the locus coeruleus and the amygdala, two brain regions suggested to be important in morphine dependence expression. Moreover, the time course of morphine-induced changes in type VIII adenylyl cyclase mRNA in locus coeruleus is related to the incidence of jumping, the most important sign of morphine withdrawal in mice. In addition, the overexpression of type VIII adenylyl cyclase mRNA in thalamic nuclei could add to morphine tolerance. These findings offer a strong support, at the molecular level, for an altered regulation of the cyclic AMP system in opiate tolerance and dependence. The present measure relates only to type VIII adenylyl cyclase mRNA and similar tests with other cyclases are needed to explore fully this relationship.
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
March 1999,
The Journal of biological chemistry,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
July 1993,
Brain research. Molecular brain research,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
August 1994,
FEBS letters,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
March 2000,
FEBS letters,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
March 1999,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
November 2000,
Molecular pharmacology,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
June 1975,
Life sciences,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
December 2001,
The Journal of biological chemistry,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
April 1994,
The Journal of biological chemistry,
I Matsuoka, and
R Maldonado, and
N Defer, and
F Noël, and
J Hanoune, and
B P Roques
July 2000,
The Journal of neuroscience : the official journal of the Society for Neuroscience,
