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A microfluorometer used for measuring intracellular free calcium has been constructed. This system was based on an XSJ-2 epifluorescence microscope with the following modifications. First, the AC mercury lamp was replaced by a DC xenon lamp to provide a stable light source. Second, an excitation filter wheel incorporating two interference filters was inserted in the light path allowing the biological sample to be excited alternately by lights of two different wavelength. The running of the whole system was under the control of a microcomputer. Such a system has successfully been used to monitor intracellular calcium changes with fluorescent calcium-sensitive dye Fura-2. Some examples of application are presented.
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
October 1996,
Archives italiennes de biologie,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
October 1965,
Medical electronics & biological engineering,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
December 1992,
IEEE transactions on bio-medical engineering,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
January 1979,
International review of cytology,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
October 1993,
International journal of radiation biology,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
March 1987,
Journal of clinical hypertension,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
October 1979,
Biochemical Society transactions,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
October 1984,
Kardiologiia,
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
January 1995,
Fiziolohichnyi zhurnal (Kiev, Ukraine : 1994),
M X Shen, and
J Zhan, and
Y L Zhao, and
L P Yang, and
X D Zhang, and
P H Zhu
January 1986,
Ciba Foundation symposium,
