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Transfection of COS-cells with a cDNA coding for human cholesterol 7 alpha-hydroxylase resulted in significant production of cholesterol 7 alpha-hydroxylase enzyme and intracellular accumulation of the product, 7 alpha-hydroxycholesterol. Presence of this enzyme activity was always associated with increased HMG CoA reductase activity. In five different independent transfection experiments resulting in a cholesterol 7 alpha-hydroxylase activity of 0.26 +/- 0.05 pmol/min/mg in the transfected cells, the HMG CoA reductase activity increased to 158 +/- 14% of that of the control cells (p < 0.01). This change was not associated with significant changes in the cholesterol content or LDL-receptor expression of the COS-cells. It is evident that the two key enzymes in cholesterol synthesis and degradation interact with each other also in extra-hepatic cells that are unable to degrade cholesterol into bile acids. Possible mechanisms for the finding is discussed.
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Y Maeda, and
M Rudling, and
K Okuda, and
I Björkhem
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E Sudjana-Sugiaman, and
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I Björkhem
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E Sudjana-Sugiaman, and
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February 1988,
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E Sudjana-Sugiaman, and
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June 1977,
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E Sudjana-Sugiaman, and
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January 1987,
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E Sudjana-Sugiaman, and
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I Björkhem
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E Sudjana-Sugiaman, and
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I Björkhem
May 1980,
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E Sudjana-Sugiaman, and
G Eggertsen, and
P Sjöblom, and
Y Maeda, and
M Rudling, and
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I Björkhem
August 1992,
Journal of lipid research,
