-transparent.png){kind=logo}
Effects of sulfhydryl compounds on cell injuries caused by hypochlorous acid (HOCl) were studied in isolated rat cardiomyocytes by way of continuous measurement of intracellular Ca2+ using calcium sensitive fluorescent dye, fura-2. Ten minutes exposure of the cell to 100 microM HOCl induced a significant increase in the intracellular free calcium concentration ([Ca2+]i) from 90 +/- 20 nM to 266 +/- 74 nM (n = 44, mean +/- S.D.). This increase in [Ca2+]i was reversed by subsequent application of dithiothreitol (DTT) in a dose dependent manner; 30 microM DTT was found effective, while 2 mM DTT almost completely restored the [Ca2+]i to the control level. Similar to DTT, cysteine ethyl ester and cysteine methyl ester could also reverse the HOCl-induced rise of [Ca2+]i where the order of the potency was DTT > cysteine ethyl ester > cysteine methyl ester. In contrast, increase in [Ca2+]i induced by HOCl was not recovered by application of other sulfhydryl compounds such as cysteine and glutathione. Since HOCl administrated in the presence of cysteine failed to induce an increase in [Ca2+]i, we conclude that membrane permeable sulfhydryl compounds may reverse the increase in [Ca2+]i caused by HOCl.
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
December 1991,
Anesthesiology,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
July 2013,
International journal of cardiology,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
October 2000,
The Journal of physiology,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
November 2006,
Annals of the New York Academy of Sciences,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
November 1991,
Experimental physiology,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
December 1998,
Anesthesiology,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
January 2010,
Biochimica et biophysica acta,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
June 2005,
Anesthesiology,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
April 1992,
European journal of pharmacology,
K Fukui, and
M Kaneda, and
E Takahashi, and
M Washio, and
K Doi
February 2000,
The Journal of physiology,
