Biomaterial Database

[Hexokinase isoenzyme II has a segment responsible for specific interaction of the enzyme with mitochondrial membranes]. 1994

N Iu Goncharova, and E V Zelenina, and L V Avramova

It was found that the ability of rat skeletal muscle hexokinase isozyme II binding to mitochondrial membranes is realized in full in the presence of Mg2+, glucose and putrescine as adsorption reagent. The hexokinase-membrane complexes obtained through the use of these reagents displayed similar stability as could be judged from their dissociation under identical conditions: either in the presence of KCl used in high concentrations or under the effect of a specific solubilization reagent--glucose-6-phosphate used in physiological concentrations. Within the composition of enzyme-membrane complexes hexokinase had the same kinetic properties which differed, however, from those of the nonbound to mitochondria enzyme. The data obtained are discussed in relation to the hexokinase isozyme II domain responsible for the specific binding of the enzyme to mitochondrial membranes and termed as the "adsorption domain". The availability of this domain is postulated in terms of the concept on the adsorption mechanism of hexokinase isozyme II activity control in skeletal muscles.

UI	MeSH Term	Description	Entries
D007425	Intracellular Membranes	Thin structures that encapsulate subcellular structures or ORGANELLES in EUKARYOTIC CELLS. They include a variety of membranes associated with the CELL NUCLEUS; the MITOCHONDRIA; the GOLGI APPARATUS; the ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.	Membranes, Intracellular,Intracellular Membrane,Membrane, Intracellular
D007527	Isoenzymes	Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.	Alloenzyme,Allozyme,Isoenzyme,Isozyme,Isozymes,Alloenzymes,Allozymes
D008931	Mitochondria, Muscle	Mitochondria of skeletal and smooth muscle. It does not include myocardial mitochondria for which MITOCHONDRIA, HEART is available.	Sarcosomes,Mitochondrion, Muscle,Muscle Mitochondria,Muscle Mitochondrion,Sarcosome
D005958	Glucosephosphates
D006593	Hexokinase	An enzyme that catalyzes the conversion of ATP and a D-hexose to ADP and a D-hexose 6-phosphate. D-Glucose, D-mannose, D-fructose, sorbitol, and D-glucosamine can act as acceptors; ITP and dATP can act as donors. The liver isoenzyme has sometimes been called glucokinase. (From Enzyme Nomenclature, 1992) EC 2.7.1.1.	Hexokinase A,Hexokinase D,Hexokinase II
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D013379	Substrate Specificity	A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.	Specificities, Substrate,Specificity, Substrate,Substrate Specificities
D051381	Rats	The common name for the genus Rattus.	Rattus,Rats, Laboratory,Rats, Norway,Rattus norvegicus,Laboratory Rat,Laboratory Rats,Norway Rat,Norway Rats,Rat,Rat, Laboratory,Rat, Norway,norvegicus, Rattus
D019298	Glucose-6-Phosphate	An ester of glucose with phosphoric acid, made in the course of glucose metabolism by mammalian and other cells. It is a normal constituent of resting muscle and probably is in constant equilibrium with fructose-6-phosphate. (Stedman, 26th ed)	Glucose 6 Phosphate