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[Comparative study of four national disinfectant testing techniques (author's transl)]. 1975

G Reybrouck, and H P Werner

Four methods for the evaluation of the bactericidal property of disinfectants have been compared. These methods are (1) the qualitative suspension test of the directives for the testing of disinfectants of the German Society for Hygiene and Microbiology, (2) the quantitative suspension test of the dutch committee on phytopharmacy, (3) the carrier or use-dilution method of the A.O.A.C. and (4) the KELSEY and SYKES' capacity test. Aim of this study has been the evaluation of (1) the repeatability and reproducibility of the test results of the four methods, and of (2) the agreement of the degree of efficiency as determined by the different techniques. At least three dilutions of the three disinfectant standards (a phenolic, an aldehyde solution and an iodophore) have been tested against Staphylococcus aureus and Pseudomonas aeruginoas. In this article the authors describe the methods used and the specific difficulties of these techniques. In the suspension technique of the DGHM, the use of only one subculture is stated as not sufficient for exact evaluation of the extinction. In the dutch suspension test, the use of pour plates leads to troubles. The preparative incubation time of the use-dilution method of the A.O.A.C. has been experienced as too long. The structure of the KELSEY and SYKES' test is complicated. Also the results by the two research-teams, are reported and summarized. It has been found that the transition from active to inactive dilution is most marked in the two suspension tests, whereas the distinction between the results of the use-dilution method of the A.O.A.C. is not so clear.

UI MeSH Term Description Entries
D007466 Iodophors Complexes of iodine and non-ionic SURFACE-ACTIVE AGENTS acting as carrier and solubilizing agent for the iodine in water. Iodophors usually enhance bactericidal activity of iodine, reduce vapor pressure and odor, minimize staining, and allow wide dilution with water. (From Merck Index, 11th ed) Tamed Iodine,Iodine, Tamed
D009426 Netherlands Country located in EUROPE. It is bordered by the NORTH SEA, BELGIUM, and GERMANY. Constituent areas are Aruba, Curacao, and Sint Maarten, formerly included in the NETHERLANDS ANTILLES. Holland,Kingdom of the Netherlands
D010636 Phenols Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D004202 Disinfectants Substances used on inanimate objects that destroy harmful microorganisms or inhibit their activity. Disinfectants are classed as complete, destroying SPORES as well as vegetative forms of microorganisms, or incomplete, destroying only vegetative forms of the organisms. They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (From Hawley's Condensed Chemical Dictionary, 11th ed) Biocide,Disinfectant,Biocides
D004353 Drug Evaluation, Preclinical Preclinical testing of drugs in experimental animals or in vitro for their biological and toxic effects and potential clinical applications. Drug Screening,Evaluation Studies, Drug, Pre-Clinical,Drug Evaluation Studies, Preclinical,Drug Evaluations, Preclinical,Evaluation Studies, Drug, Preclinical,Evaluation, Preclinical Drug,Evaluations, Preclinical Drug,Medicinal Plants Testing, Preclinical,Preclinical Drug Evaluation,Preclinical Drug Evaluations,Drug Screenings,Screening, Drug,Screenings, Drug
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005860 Germany, West The former Federal Republic of Germany which was reunified with the former German Democratic Republic in 1990. Federal Republic of Germany,Germany, Federal Republic of
D006113 United Kingdom Country in northwestern Europe including Great Britain and the northern one-sixth of the island of Ireland, located between the North Sea and north Atlantic Ocean. The capital is London. Great Britain,Isle of Man
D000447 Aldehydes Organic compounds containing a carbonyl group in the form -CHO. Aldehyde

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