[The meaning of the results of four national disinfectant testing techniques (author's transl)]. 1975

G Reybrouck, and H van de Voorde

The bactericidal activity of three disinfectant standards has been determined by four national methods (the qualitative suspension test of the German Society for Hygiene and Microbiology DGHM, the quantitative suspension test of the Dutch Committee on Phytopharmacy, the use-dilution method of the A.O.A.C. and the Kelsey and Sykes' test) in order to compare these four testing techniques. In previous publications the results of these experiments and the degree of standardisation of the four methods have been tested. The purpose of the present study is to evaluate to what extent the results obtained by the four testing techniques do correspond. The bactericidal activity can be expressed as a germicidal effect. In theory (Tab. 1) the values of germicidal effect can be similar for the four testing techniques. In practice (Tab. 2 and 3) it has been found that with the suspension technique of the DGHM systematically higher values are obtained than in the other tests. These can not be compared to each other, because the medication times differ. Although the values of the germicidal effect obtained are widely different, it can happen that the minimum effective concentration determined by the four methods be identical as each of these methods applies different criteria. The authors have therefore calculated the minimum effective concentration which is required for the disinfectant standard by the four disinfectant testing techniques (Tab. 4). Even here, no correspondence has been found between the four methods. The lethal concentration of the disinfectant standards determined by the use-dilution method of the A.O.A.C. is thus 2 to 16 times those required by the Dutch suspension test. The difference in effective concentrations, however, is not constant and is not related to the type of disinfectant or the test organism. In consequence, there is no way of establishing a comparison between the results of the four methods with a view to setting up a conversion table. Each disinfectant testing technique has its own characteristics.

UI MeSH Term Description Entries
D009426 Netherlands Country located in EUROPE. It is bordered by the NORTH SEA, BELGIUM, and GERMANY. Constituent areas are Aruba, Curacao, and Sint Maarten, formerly included in the NETHERLANDS ANTILLES. Holland,Kingdom of the Netherlands
D011550 Pseudomonas aeruginosa A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection. Bacillus aeruginosus,Bacillus pyocyaneus,Bacterium aeruginosum,Bacterium pyocyaneum,Micrococcus pyocyaneus,Pseudomonas polycolor,Pseudomonas pyocyanea
D004202 Disinfectants Substances used on inanimate objects that destroy harmful microorganisms or inhibit their activity. Disinfectants are classed as complete, destroying SPORES as well as vegetative forms of microorganisms, or incomplete, destroying only vegetative forms of the organisms. They are distinguished from ANTISEPTICS, which are local anti-infective agents used on humans and other animals. (From Hawley's Condensed Chemical Dictionary, 11th ed) Biocide,Disinfectant,Biocides
D004353 Drug Evaluation, Preclinical Preclinical testing of drugs in experimental animals or in vitro for their biological and toxic effects and potential clinical applications. Drug Screening,Evaluation Studies, Drug, Pre-Clinical,Drug Evaluation Studies, Preclinical,Drug Evaluations, Preclinical,Evaluation Studies, Drug, Preclinical,Evaluation, Preclinical Drug,Evaluations, Preclinical Drug,Medicinal Plants Testing, Preclinical,Preclinical Drug Evaluation,Preclinical Drug Evaluations,Drug Screenings,Screening, Drug,Screenings, Drug
D005069 Evaluation Studies as Topic Works about studies that determine the effectiveness or value of processes, personnel, and equipment, or the material on conducting such studies. Critique,Evaluation Indexes,Evaluation Methodology,Evaluation Report,Evaluation Research,Methodology, Evaluation,Pre-Post Tests,Qualitative Evaluation,Quantitative Evaluation,Theoretical Effectiveness,Use-Effectiveness,Critiques,Effectiveness, Theoretical,Evaluation Methodologies,Evaluation Reports,Evaluation, Qualitative,Evaluation, Quantitative,Evaluations, Qualitative,Evaluations, Quantitative,Indexes, Evaluation,Methodologies, Evaluation,Pre Post Tests,Pre-Post Test,Qualitative Evaluations,Quantitative Evaluations,Report, Evaluation,Reports, Evaluation,Research, Evaluation,Test, Pre-Post,Tests, Pre-Post,Use Effectiveness
D005860 Germany, West The former Federal Republic of Germany which was reunified with the former German Democratic Republic in 1990. Federal Republic of Germany,Germany, Federal Republic of
D006113 United Kingdom Country in northwestern Europe including Great Britain and the northern one-sixth of the island of Ireland, located between the North Sea and north Atlantic Ocean. The capital is London. Great Britain,Isle of Man
D013211 Staphylococcus aureus Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.
D014481 United States A country in NORTH AMERICA between CANADA and MEXICO.

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