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By the use of ferritin-conjugated antibody (conjugate) indirect immunoelectron microscopy, NADPH-cytochrome c reductase was localized on rat liver microsomes. Most microsomes in the sections had from 1 to 12 conjugates on their outer surfaces. Among the conjugates, 83% was estimated to bind to NADPH-cytochrome c reductase at a molecular ratio of 1:1, 12% at the ratio of 2:1, and 5% at the ratio of 3 or 4:1. The correlation between immunochemical and morphological data confirmed that most of the NADPH-cytochrome c reducatase reacted with the conjugates. Subsequent morphological analyses have revealed that the enzyme is distributed homogeneously on the outer surfaces of microsomes but heterogeneously within microsomes in groups of three to five enzyme molecules.
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
January 1975,
Biochimica et biophysica acta,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
August 1977,
Biochemical and biophysical research communications,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
March 1978,
Life sciences,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
February 1982,
Biochemical pharmacology,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
August 1972,
Biochemical and biophysical research communications,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
June 1972,
Biochemical and biophysical research communications,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
April 1975,
Biochimica et biophysica acta,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
February 1992,
Biochimica et biophysica acta,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
April 1971,
Biochimica et biophysica acta,
T Morimoto, and
S Matsuura, and
S Sasaki, and
Y Yashiro, and
T Omura
August 1978,
The Journal of cell biology,
