Biomaterial Database

The development of antibody to Cowdria ruminantium in mice and its role in heartwater disease. 1993

B Byrom, and S M Mahan, and A F Barbet

University of Florida/USAID/SADCC Heartwater Research Project, Causeway, Zimbabwe.

DBA/2 and Balb/c mice were used as a model to study the immune responses to Cowdria ruminantium. Both strains of mice were inoculated with 1, 10 or 100 LD50 of the Crystal Springs strain of C. ruminantium. Antibody to C. ruminantium started to develop in the second week post-inoculation and the titre of the antibody was dependent on the inoculation dose of C. ruminantium. The possible role of antibody in heartwater disease was studied by in vitro neutralization assays using both mouse sera and bovine sera. Results of these tests show that hyperimmune serum from both DBA/2 and Balb/c mice had the ability to neutralize infection in vitro, with the DBA/2 serum showing a greater neutralizing effect. Two bovine sera, one from a laboratory infected animal and one a pool from two animals infected in the field also gave a neutralizing effect.

UI	MeSH Term	Description	Entries
D008807	Mice, Inbred BALB C	An inbred strain of mouse that is widely used in IMMUNOLOGY studies and cancer research.	BALB C Mice, Inbred,BALB C Mouse, Inbred,Inbred BALB C Mice,Inbred BALB C Mouse,Mice, BALB C,Mouse, BALB C,Mouse, Inbred BALB C,BALB C Mice,BALB C Mouse
D008811	Mice, Inbred DBA	An inbred strain of mouse. Specific substrains are used in a variety of areas of BIOMEDICAL RESEARCH such as DBA/1J, which is used as a model for RHEUMATOID ARTHRITIS.	Mice, DBA,Mouse, DBA,Mouse, Inbred DBA,DBA Mice,DBA Mice, Inbred,DBA Mouse,DBA Mouse, Inbred,Inbred DBA Mice,Inbred DBA Mouse
D009500	Neutralization Tests	The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50).	Neutralization Test,Test, Neutralization,Tests, Neutralization
D006357	Heartwater Disease	A tick-borne septicemic disease of domestic and wild ruminants caused by EHRLICHIA RUMINANTIUM.	Disease, Heartwater
D000818	Animals	Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA.	Animal,Metazoa,Animalia
D000907	Antibodies, Bacterial	Immunoglobulins produced in a response to BACTERIAL ANTIGENS.	Bacterial Antibodies
D016995	Ehrlichia ruminantium	A species of gram-negative bacteria in the family ANAPLASMATACEAE, that causes HEARTWATER DISEASE in ruminants.	Cowdria ruminantium
D051379	Mice	The common name for the genus Mus.	Mice, House,Mus,Mus musculus,Mice, Laboratory,Mouse,Mouse, House,Mouse, Laboratory,Mouse, Swiss,Mus domesticus,Mus musculus domesticus,Swiss Mice,House Mice,House Mouse,Laboratory Mice,Laboratory Mouse,Mice, Swiss,Swiss Mouse,domesticus, Mus musculus