BIOMAT Database Logo BIOMAT Database Logo

Identification of a human delta opioid receptor: cloning and expression. 1994

R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Department of Pharmacology, University of Arizona College of Medicine, Tucson 85724.

The delta opioid receptor is an important target for analgesic drug development. This report describes the identification of delta opioid receptor clones from human cDNA libraries and the preparation of a human delta receptor cDNA in the pcDNA3 expression vector for transfection studies. The cDNA encodes a 372 amino acid protein that has 93% amino acid identity to mouse and rat delta receptors. COS-7 cells transfected with this clone express over 1.0 pmol receptor/mg protein when measured by saturation binding with [3H]naltrindole. The delta receptor selective ligands NTB, BNTX, [4'-Cl-Phe4]DPDPE and [D-Ala2,Glu4]deltorphin all have Ki values under 10 nM while the affinities of the mu and kappa opioid receptor ligands CTAP and U-69593, respectively, are over 4.0 microM. Agonists show binding to multiple affinity states of the receptor consistent with the presence of G-protein coupled and uncoupled forms of the expressed receptor. The 8-fold higher affinity of NTB relative to BNTX suggests that the human delta receptor is of the delta 2 subtype.

UI MeSH Term Description Entries
D008969 Molecular Sequence Data Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009271 Naltrexone Derivative of noroxymorphone that is the N-cyclopropylmethyl congener of NALOXONE. It is a narcotic antagonist that is effective orally, longer lasting and more potent than naloxone, and has been proposed for the treatment of heroin addiction. The FDA has approved naltrexone for the treatment of alcohol dependence. Antaxone,Celupan,EN-1639A,Nalorex,Naltrexone Hydrochloride,Nemexin,ReVia,Trexan,EN 1639A,EN1639A
D009292 Narcotic Antagonists Agents inhibiting the effect of narcotics on the central nervous system. Competitive Opioid Antagonist,Narcotic Antagonist,Opioid Antagonist,Opioid Antagonists,Opioid Receptor Antagonist,Opioid Reversal Agent,Competitive Opioid Antagonists,Opioid Receptor Antagonists,Opioid Reversal Agents,Agent, Opioid Reversal,Agents, Opioid Reversal,Antagonist, Competitive Opioid,Antagonist, Narcotic,Antagonist, Opioid,Antagonist, Opioid Receptor,Antagonists, Competitive Opioid,Antagonists, Narcotic,Antagonists, Opioid,Antagonists, Opioid Receptor,Opioid Antagonist, Competitive,Opioid Antagonists, Competitive,Receptor Antagonist, Opioid,Receptor Antagonists, Opioid,Reversal Agent, Opioid,Reversal Agents, Opioid
D009842 Oligopeptides Peptides composed of between two and twelve amino acids. Oligopeptide
D002460 Cell Line Established cell cultures that have the potential to propagate indefinitely. Cell Lines,Line, Cell,Lines, Cell
D003001 Cloning, Molecular The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells. Molecular Cloning
D004745 Enkephalins One of the three major families of endogenous opioid peptides. The enkephalins are pentapeptides that are widespread in the central and peripheral nervous systems and in the adrenal medulla. Enkephalin
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000595 Amino Acid Sequence The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION. Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia

Related Publications

R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Cloning of a delta opioid receptor by functional expression.
December 1992, Science (New York, N.Y.),
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Molecular cloning and expression of a delta-opioid receptor from rat brain.
April 1994, Journal of neuroscience research,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
The human delta-opioid receptor: genomic organization, cDNA cloning, functional expression, and distribution in human brain.
December 1994, Molecular pharmacology,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization.
February 1994, Proceedings of the National Academy of Sciences of the United States of America,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
The delta-opioid receptor: isolation of a cDNA by expression cloning and pharmacological characterization.
December 1992, Proceedings of the National Academy of Sciences of the United States of America,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Cloning and characterization of a delta opioid receptor from zebrafish.
November 1998, Biochemical Society transactions,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Expression cloning of a full-length cDNA encoding delta opioid receptor from mouse thymocytes.
April 1996, Journal of neuroimmunology,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Relative efficacies of delta-opioid receptor agonists at the cloned human delta-opioid receptor.
May 1997, European journal of pharmacology,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Cloning, expression, and crystallization of the V delta domain of a human gamma delta T-cell receptor.
December 1996, Protein science : a publication of the Protein Society,
R J Knapp, and E Malatynska, and L Fang, and X Li, and E Babin, and M Nguyen, and G Santoro, and E V Varga, and V J Hruby, and W R Roeske
Immunofluorescent identification of a delta (delta)-opioid receptor on primary afferent nerve terminals.
December 1993, Neuroreport,
Copied contents to your clipboard!