BIOMAT Database Logo BIOMAT Database Logo

Quantitation of urinary 1,2,3,4-tetrahydro-beta-carboline and 1-methyl-1,2,3,4-tetrahydro-beta-carboline by high-performance liquid chromatography. 1994

H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Department of Dental Pharmacology, Asahi University School of Dentistry, Gifu, Japan.

A high-performance liquid chromatographic method was developed to quantify 1,2,3,4-tetrahydro-beta-carboline (TBC) and 1-methyl-1,2,3,4-tetrahydro-beta-carboline (MTBC) in human urine. Urine samples with added internal standard were subjected to a reaction with fluorescamine and solvent extractions to remove the precursor tryptamine, which readily condenses with aldehydes in samples and reagents. Such a pretreatment completely suppressed the artifactual formation of TBC and MTBC during analytical procedures. The purified original tetrahydro-beta-carbolines and the internal standard were separated by reversed-phase ion-pair chromatography with fluorescent detection. Their simultaneous separation was automatically completed in a short time (< 12 min). Both TBC and MTBC were quantified at ng/mL concentrations. The quantitative results revealed a wide variation in urinary levels of TBC and MTBC, possibly indicating that their considerable amounts excreted in the urine originate from dietary sources.

UI MeSH Term Description Entries
D007202 Indicators and Reagents Substances used for the detection, identification, analysis, etc. of chemical, biological, or pathologic processes or conditions. Indicators are substances that change in physical appearance, e.g., color, at or approaching the endpoint of a chemical titration, e.g., on the passage between acidity and alkalinity. Reagents are substances used for the detection or determination of another substance by chemical or microscopical means, especially analysis. Types of reagents are precipitants, solvents, oxidizers, reducers, fluxes, and colorimetric reagents. (From Grant & Hackh's Chemical Dictionary, 5th ed, p301, p499) Indicator,Reagent,Reagents,Indicators,Reagents and Indicators
D008297 Male Males
D002243 Carbolines A group of pyrido-indole compounds. Included are any points of fusion of pyridine with the five-membered ring of indole and any derivatives of these compounds. These are similar to CARBAZOLES which are benzo-indoles. Carboline,Pyrido(4,3-b)Indole,Beta-Carbolines,Pyrido(4,3-b)Indoles,Beta Carbolines
D002851 Chromatography, High Pressure Liquid Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed. Chromatography, High Performance Liquid,Chromatography, High Speed Liquid,Chromatography, Liquid, High Pressure,HPLC,High Performance Liquid Chromatography,High-Performance Liquid Chromatography,UPLC,Ultra Performance Liquid Chromatography,Chromatography, High-Performance Liquid,High-Performance Liquid Chromatographies,Liquid Chromatography, High-Performance
D005260 Female Females
D005450 Fluorescamine A nonfluorescent reagent for the detection of primary amines, peptides and proteins. The reaction products are highly fluorescent. Fluram
D006801 Humans Members of the species Homo sapiens. Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D000293 Adolescent A person 13 to 18 years of age. Adolescence,Youth,Adolescents,Adolescents, Female,Adolescents, Male,Teenagers,Teens,Adolescent, Female,Adolescent, Male,Female Adolescent,Female Adolescents,Male Adolescent,Male Adolescents,Teen,Teenager,Youths
D000328 Adult A person having attained full growth or maturity. Adults are of 19 through 44 years of age. For a person between 19 and 24 years of age, YOUNG ADULT is available. Adults
D013050 Spectrometry, Fluorescence Measurement of the intensity and quality of fluorescence. Fluorescence Spectrophotometry,Fluorescence Spectroscopy,Spectrofluorometry,Fluorescence Spectrometry,Spectrophotometry, Fluorescence,Spectroscopy, Fluorescence

Related Publications

H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Identification and measurement of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline by gas chromatography-mass spectrometry.
August 1982, Biochemical pharmacology,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Metabolic hydroxylation of 1-methyl-1,2,3,4-tetrahydro-beta-carboline in humans.
December 1995, Pharmacology, biochemistry, and behavior,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Analysis of 1-methyl-1,2,3,4-tetrahydro-beta-carboline in alcoholic beverages.
April 1981, Food and cosmetics toxicology,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
High-performance liquid chromatography-electrospray ionisation-tandem mass spectrometry for the analysis of 1,2,3,4-tetrahydro-beta-carboline derivatives.
April 1997, Journal of chromatography. A,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Identification and quantification of 1,2,3,4-tetrahydro-beta-carboline, 2-methyl-1,2,3,4-tetrahydro-beta-carboline, and 6-methoxy-1,2,3,4-tetrahydro-beta-carboline as in vivo constituents of rat brain and adrenal gland.
January 1981, Biochemical pharmacology,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Distribution of 1,2,3,4-tetrahydro-beta-carboline and 6-methoxy-1,2,3,4-tetrahydro-beta-carboline in mice.
May 1984, Acta pharmacologica et toxicologica,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Antioxidative activity of 1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid.
September 1999, Arzneimittel-Forschung,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
1-methyl-1,2,3,4-tetrahydro-beta-carboline-3-carboxylic acid and 1,2, 3,4-tetrahydro-beta-carboline-3-carboxylic acid in fruits.
December 1999, Journal of agricultural and food chemistry,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
Synthesis of 1,2,3,4-tetrahydro-beta-carboline derivatives as hepatoprotective agents. III. Introduction of substituents onto methyl 1,2,3,4-tetrahydro-beta-carboline-2-carbodithioate.
August 1987, Chemical & pharmaceutical bulletin,
H Tsuchiya, and S Ohtani, and K Yamada, and N Takagi, and H Todoriki, and T Hayashi
[The reduction of 1,2,3,4-tetrahydro-1-oxo-beta-carboline].
June 1968, Acta pharmaceutica Hungarica,
Copied contents to your clipboard!