-transparent.png){kind=logo}
The natural arrest of vertebrate unfertilized eggs in second meiotic metaphase results from the activity of cytostatic factor (CSF). The product of the c-mos(xe) proto-oncogene is thought to be a component of CSF and can induce metaphase arrest when injected into blastomeres of two-cell embryos. The c-Mos(xe) protein can directly activate the mitogen-activated protein kinase kinase (MAP kinase kinase) in vitro, leading to activation of MAP kinase. MAP kinase and c-Mos(xe) are active in unfertilized eggs and are rapidly inactivated after fertilization. Microinjection of thiophosphorylated MAP kinase into one blastomere of a two-cell embryo induced metaphase arrest similar to that induced by c-Mos(xe). However, only arrest with c-Mos(xe) was associated with activation of endogenous MAP kinase. These results indicate that active MAP kinase is a component of CSF in Xenopus and suggest that the CSF activity of c-Mos(xe) is mediated by MAP kinase.
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
November 1999,
Science (New York, N.Y.),
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
April 1995,
Developmental biology,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
July 1995,
Nature,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
August 1990,
The Journal of cell biology,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
August 1992,
Molecular reproduction and development,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
June 1995,
The EMBO journal,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
February 1993,
The Journal of biological chemistry,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
June 1994,
Developmental biology,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
January 1993,
Developmental genetics,
O Haccard, and
B Sarcevic, and
A Lewellyn, and
R Hartley, and
L Roy, and
T Izumi, and
E Erikson, and
J L Maller
September 2001,
Biology of the cell,
