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We have analyzed the DNA sequence requirements for the functioning of TATA elements by examining the transcriptional activities associated with 24 promoters, including representatives of each of the 21 point mutations in the consensus sequence from plants, TATATATA, in a HeLa in vitro system and in a chimeric in vitro system in which human TATA-binding protein (hTBP) was replaced by purified TBP of Arabidopsis (aTBP-1). Although the relative transcriptional activities varied among these promoters, both systems gave virtually identical results. Among the mutant TATA elements, those with the sequences TAGAGATA and GAGAGAGA had undetectable activity. The rest had activities that ranged from 7% to 130% of the activity associated with the consensus element. These results suggest the functional conservation of TBP between plants and animals.
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
December 2002,
Molecular and cellular biology,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
January 1996,
Methods in enzymology,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
August 1990,
Molecular and cellular biology,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
July 1988,
Nature,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
May 2001,
The Journal of biological chemistry,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
September 1993,
Proceedings of the National Academy of Sciences of the United States of America,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
May 1994,
Bioscience, biotechnology, and biochemistry,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
February 1992,
Proceedings of the National Academy of Sciences of the United States of America,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
April 1993,
Molecular and cellular biology,
F Mukumoto, and
S Hirose, and
H Imaseki, and
K Yamazaki
January 2000,
The Journal of biological chemistry,
