BIOMAT Database Logo BIOMAT Database Logo

Immunogenicity and antigenicity of a Plasmodium falciparum protein fraction (90-110 kDa) able to protect squirrel monkeys against asexual blood stages. 1994

S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Unité de Parasitologie Expérimentale, Institut Pasteur, Paris, France.

A monkey vaccination trial using a Plasmodium falciparum protein fraction containing antigens of 90-110 kDa is reported. The fraction was obtained by electroelution from preparative polyacrylamide gels. Three monkeys out of five resisted a heavy challenge dose of highly virulent parasites. Using specific antisera, several components of the fraction were identified, namely heat shock protein 90 (hsp90), Ag44/RhopH3, ABRA, 96tR/GBP130 and Pf96 protease. The fraction did not contain KAHRP, nor the SERP antigen. The antibody response of the monkeys was studied on these individual antigens purified by preparative immunoprecipitation. Surprisingly, hsp90 was found in the immunoprecipitates obtained with SERP antisera. Interestingly, the response to hsp90 correlated with protection, high antibody titres being found only in the protected monkeys. In contrast, no correlation with protection could be found for the response to the other antigens.

UI MeSH Term Description Entries
D010963 Plasmodium falciparum A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics. Plasmodium falciparums,falciparums, Plasmodium
D011233 Precipitin Tests Serologic tests in which a positive reaction manifested by visible CHEMICAL PRECIPITATION occurs when a soluble ANTIGEN reacts with its precipitins, i.e., ANTIBODIES that can form a precipitate. Precipitin Test,Test, Precipitin,Tests, Precipitin
D004591 Electrophoresis, Polyacrylamide Gel Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. Polyacrylamide Gel Electrophoresis,SDS-PAGE,Sodium Dodecyl Sulfate-PAGE,Gel Electrophoresis, Polyacrylamide,SDS PAGE,Sodium Dodecyl Sulfate PAGE,Sodium Dodecyl Sulfate-PAGEs
D004797 Enzyme-Linked Immunosorbent Assay An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed. ELISA,Assay, Enzyme-Linked Immunosorbent,Assays, Enzyme-Linked Immunosorbent,Enzyme Linked Immunosorbent Assay,Enzyme-Linked Immunosorbent Assays,Immunosorbent Assay, Enzyme-Linked,Immunosorbent Assays, Enzyme-Linked
D006360 Heat-Shock Proteins Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions. Stress Protein,Stress Proteins,Heat-Shock Protein,Heat Shock Protein,Heat Shock Proteins,Protein, Stress
D000818 Animals Unicellular or multicellular, heterotrophic organisms, that have sensation and the power of voluntary movement. Under the older five kingdom paradigm, Animalia was one of the kingdoms. Under the modern three domain model, Animalia represents one of the many groups in the domain EUKARYOTA. Animal,Metazoa,Animalia
D000913 Antibodies, Protozoan Immunoglobulins produced in a response to PROTOZOAN ANTIGENS. Protozoan Antibodies
D012453 Saimiri A genus of the family CEBIDAE consisting of four species: S. boliviensis, S. orstedii (red-backed squirrel monkey), S. sciureus (common squirrel monkey), and S. ustus. They inhabit tropical rain forests in Central and South America. S. sciureus is used extensively in research studies. Monkey, Squirrel,Squirrel Monkey,Monkeys, Squirrel,Saimirus,Squirrel Monkeys
D015153 Blotting, Western Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes. Immunoblotting, Western,Western Blotting,Western Immunoblotting,Blot, Western,Immunoblot, Western,Western Blot,Western Immunoblot,Blots, Western,Blottings, Western,Immunoblots, Western,Immunoblottings, Western,Western Blots,Western Blottings,Western Immunoblots,Western Immunoblottings
D015800 Protozoan Proteins Proteins found in any species of protozoan. Proteins, Protozoan

Related Publications

S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Failure of a synthetic vaccine to protect Aotus lemurinus against asexual blood stages of Plasmodium falciparum.
November 1992, The American journal of tropical medicine and hygiene,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Recombinant protein vaccines against the asexual blood stages of Plasmodium falciparum.
January 2010, Human vaccines,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Protective immunization of the squirrel monkey against asexual blood stages of Plasmodium falciparum by use of parasite protein fractions.
January 1984, Proceedings of the National Academy of Sciences of the United States of America,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Immunization against Plasmodium falciparum asexual blood stages using soluble antigens.
April 1984, Clinical and experimental immunology,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Activity of Epigenetic Inhibitors against Plasmodium falciparum Asexual and Sexual Blood Stages.
June 2020, Antimicrobial agents and chemotherapy,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Studies in owl monkeys leading to the development of a synthetic vaccine against the asexual blood stages of Plasmodium falciparum.
October 1990, The American journal of tropical medicine and hygiene,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
Ultrastructure of the asexual blood stages of Plasmodium falciparum.
January 2010, Methods in cell biology,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
In-vitro activity of primaquine against the asexual blood stages of Plasmodium falciparum.
March 1999, Annals of tropical medicine and parasitology,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
In vitro activity of fluorescent dyes against asexual blood stages of Plasmodium falciparum.
November 2012, Antimicrobial agents and chemotherapy,
S Bonnefoy, and J Gysin, and T Blisnick, and M Guillotte, and B Carcy, and L Pereira da Silva, and O Mercereau-Puijalon
The Pf332 gene codes for a megadalton protein of Plasmodium falciparum asexual blood stages.
January 1992, Memorias do Instituto Oswaldo Cruz,
Copied contents to your clipboard!