Biomaterial Database

Isolation of cysteine proteinase inhibitor, cystatin A, from human nails. 1993

H Tsushima

Department of Hygiene, Kawasaki Medical School, Okayama, Japan.

A cysteine proteinase inhibitor was found in human nail extract treated with 0.01 M Tris HCl buffer, pH 8.0. It had a 2-fold lower and a 4.5-fold higher activity than that of human skin and hair extracts, respectively. From 5.9 g of human nail, 0.1 mg of cysteine proteinase inhibitor was obtained. It was purified by sequential DE-52 ion exchange and carboxymethyl papain affinity chromatography. The purified inhibitor had an apparent molecular mass of 12 kDa as determined by sodium dodecyl sulphate polyacrylamide gel electrophoresis. It was more stable against heat and pH than most other proteins. Immunologically, it had the same antigenicity compared with human epidermal cystatin A. Its N-terminal amino acid sequence showed a mixed form comprising a full-length MIPG sequence a truncated IPGG sequence. This sequence was identical to human cystatin A consisting of 20% of the full-length and 80% of the truncated form. These results showed that human nail also contains cystatin A type cysteine proteinase inhibitor. Nails can be used as a source of cystatin A.

UI	MeSH Term	Description	Entries
D008969	Molecular Sequence Data	Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.	Sequence Data, Molecular,Molecular Sequencing Data,Data, Molecular Sequence,Data, Molecular Sequencing,Sequencing Data, Molecular
D009262	Nails	The thin, horny plates that cover the dorsal surfaces of the distal phalanges of the fingers and toes of primates.	Fingernails,Toenails,Fingernail,Nail,Toenail
D006801	Humans	Members of the species Homo sapiens.	Homo sapiens,Man (Taxonomy),Human,Man, Modern,Modern Man
D006863	Hydrogen-Ion Concentration	The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH	pH,Concentration, Hydrogen-Ion,Concentrations, Hydrogen-Ion,Hydrogen Ion Concentration,Hydrogen-Ion Concentrations
D000595	Amino Acid Sequence	The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.	Protein Structure, Primary,Amino Acid Sequences,Sequence, Amino Acid,Sequences, Amino Acid,Primary Protein Structure,Primary Protein Structures,Protein Structures, Primary,Structure, Primary Protein,Structures, Primary Protein
D015891	Cystatins	A homologous group of endogenous CYSTEINE PROTEINASE INHIBITORS. The cystatins inhibit most CYSTEINE ENDOPEPTIDASES such as PAPAIN, and other peptidases which have a sulfhydryl group at the active site.	Cystatin,Cystatin Superfamily,Stefin,Cystatin-Related Proteins,Stefins,Type 1 Cystatins,Type 2 Cystatins,Type 3 Cystatins,Type I Cystatins,Type II Cystatins,Type III Cystatins,Cystatin Related Proteins,Cystatins, Type 1,Cystatins, Type 2,Cystatins, Type 3,Cystatins, Type I,Cystatins, Type II,Cystatins, Type III